Najafgarh的排水沟在亚穆纳河的污染中起着重要的作用,占污染总量的40%。因此,调查和分析微生物多样性至关重要,代谢功能能力,和Najafgarh排水沟中存在的抗生素抗性基因(ARGs)。此外,研究水质及其与排水沟中微生物增殖的关系至关重要。获得的结果证实了水质恶化的物理化学参数,如生化需氧量(BOD),化学需氧量(COD),溶解氧(DO),和总悬浮固体(TSS)在125-140、400-460、0-0.2、25-140.4mg/l范围内,分别违反了标准允许的国家标准和全球标准。此外,下一代测序(NGS)分析证实了Thauera等属的存在,弓形虫,假单胞菌,Geobacter,脱氯单胞菌,Tolumonas,硫螺旋藻,Desulfovibrio,气单胞菌,拟杆菌,普雷沃氏菌,回肠杆菌,双歧杆菌,梭菌等.从Najafgarh排水沟中获得的废水中还有864种ARG。研究结果证实,从该数据集中报告的致病物种对动物和人类健康具有严重的有害影响。Further,皮尔逊的r相关分析表明,环境变量,主要是总溶解固体(TDS)和化学需氧量(COD),在驱动这个严重污染的下水道的微生物群落结构中起着关键作用。因此,水质差,微生物联系的存在,致病标志物,整个排水口的ARGs证实,在不久的将来,这将是致病因子(病原体)向家庭和饮用水供应传播的一个潜在原因。
The Najafgarh drain plays a significant role in the pollution of the Yamuna River, accounting for 40% of the total pollution. Therefore, it is crucial to investigate and analyze the microbial diversity, metabolic functional capacity, and antibiotic resistance genes (ARGs) present in the Najafgarh drain. Additionally, studying the water quality and its relationship with the proliferation of microorganisms in the drain is of utmost importance. Results obtained confirmed the deteriorated water quality as physico-chemical parameters such as biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), and total suspended solids (TSS) in the range of 125-140, 400-460, 0-0.2, 25-140.4 mg/l respectively violated the standard permissible national and global standards. In addition, the next generation sequencing (NGS) analysis confirm the presence of genus such as Thauera, Arcobacter, Pseudomonas, Geobacter, Dechloromonas, Tolumonas, Sulfurospirullum, Desulfovibrio, Aeromonas, Bacteroides, Prevotella, Cloacibacterium, Bifidobacterium, Clostridium etc. along with 864 ARGs in the wastewater obtained from the Najafgarh drain. Findings confirm that the pathogenic species reported from this dataset possess severe detrimental impact on faunal and human health. Further, Pearson\'s r correlation analysis indicated that environmental variables, mainly total dissolved solids (TDS) and chemical oxygen demand (COD), play a pivotal role in driving microbial community structure of this heavily polluted drain. Thus, the poor water quality, presence of a microbial nexus, pathogenic markers, and ARGs throughout this drain confirmed that it would be one potential contributor to the dissemination of disease-causing agents (pathogens) to the household and drinking water supplies in the near future.