背景:背景最近,一个实时系统,命名为癌症诊断探针(CDP),已开发用于诊断乳腔侧边缘中肿瘤前/肿瘤细胞的存在。检测机制是实时测定癌细胞或非典型细胞释放的ROS/H2O2,通过逆转Warburg效应和缺氧糖酵解途径。
目标:这里,我们设计了一项基于CDP对39例新辅助乳腺癌病例的387例内切缘(IM)进行实时检查的人体模型研究.材料和方法:通过输入的针头传感器检查每个病变,并记录电评分。每个测试病变的永久性病理结果是我们评估CDP评分的金标准。将CDP结果与肿瘤侧缘的永久性病理学进行比较(作为常规的边缘评估程序)。
结果:结果表明,CDP对这些病例的腔侧边缘评分的敏感性为91%。CDP检测到的总共18例涉及IM,在肿瘤侧样本的病理切片中被宣布为游离边缘。CDP仅错过了五个涉及的IM。
结论:这种敏感性表明,基于代谢(此处:缺氧糖酵解)的癌细胞示踪在清晰和相关的腔侧边缘评估之间显示出不同的电化学响应。
结论:这项人体研究显示CDP在新辅助病例的BCS后获得清晰的边缘具有有希望的作用。
BACKGROUND: Background Recently, a real-time system, named cancer diagnostic probe (CDP), has been developed to diagnose the presence of pre-neoplastic/neoplastic cells in breast cavity side margins. Detecting mechanism is real-time determination of the ROS/H2 O2 released from cancer or atypical cells, through reverse Warburg effect and hypoxia glycolysis pathways.
OBJECTIVE: Here, we designed a human model study based on real-time checking of 387 internal margins (IM) from 39 neoadjuvant breast cancer cases by CDP. MATERIALS & METHODS: Each lesion was checked by entered needle sensor and electrical scores were recorded. The permanent pathology result of each tested lesion was our gold standard to evaluate CDP scoring. CDP results were compared with permanent pathology of tumour side margins (as a conventional margin evaluation procedure).
RESULTS: Results showed that the sensitivity of CDP in scoring the cavity side margins of those cases is 91%. A total of 18 involved IM which had been detected by CDP were declared as free margins in pathology section of tumour side samples. Just five involved IM were missed by CDP.
CONCLUSIONS: Such sensitivity revealed that metabolism based (here: hypoxia glycolysis) tracing of cancer cells show distinct electrochemical responses between clear and involved cavity side margin evaluation.
CONCLUSIONS: This human study showed the promising role of CDP to achieve clear margins after BCS of neoadjuvant cases.