胃癌仍然是世界上最普遍和高度致命的疾病。MAP4K4是Ste20的成员,在各种病理中起着重要作用,包括癌症.然而,其在胃癌中的作用尚未完全阐明。因此,本研究旨在确定MAP4K4在胃癌中的促瘤作用及其是否可作为一种新的、可靠的生物标志物来预测胃癌的预后。为此,根据MAP4K4的表达水平将样本分为高表达组和低表达组。使用Kaplan-Meier生存分析评估MAP4K4表达与预后的关联。此外,使用ESTIMATE进行免疫浸润分析以评估样品的肿瘤免疫评分。
这些发现揭示了MAP4K4在肿瘤样品中的表达显著高于在邻近样品中的表达。高表达组显著富集肿瘤相关通路,如PI3K-Akt信号通路。此外,免疫浸润分析显示免疫评分与MAP4K4表达呈正相关。我们还观察到miRNA,例如miR-192-3p(R=-0.317,p值3.111×10-9),miR-33b-5p(R=-0.238,p值1.166×10-5),和miR-582-3p(R=-0.214,p值8.430×10-5),对MAP4K4有潜在的负调节作用。此外,我们确定了几个转录因子,泛素化蛋白质,和可能调节MAP4K4的相互作用蛋白。还鉴定了MAP4K4与DNA甲基化之间的关系。最后,我们验证了MAP4K4的高表达及其促癌作用。
MAP4K4可能与胃癌的进展密切相关,入侵,和转移。它的高表达对胃癌患者的预后有负面影响。这表明MAP4K4是胃癌的重要预后因子,可作为一个新的潜在预后检测和治疗靶点。
Gastric cancer remains the most prevalent and highly lethal disease worldwide. MAP4K4, a member of Ste20, plays an important role in various pathologies, including cancer. However, its role in gastric cancer is not yet fully elucidated. Therefore, this study aims to determine the tumor-promoting role of MAP4K4 in gastric cancer and whether it can be used as a new and reliable biomarker to predict the prognosis of gastric cancer. For this purpose, we divide the samples into high- and low-expression groups according to the expression level of MAP4K4. The association of MAP4K4 expression with prognosis is assessed using the Kaplan-Meier survival analysis. Furthermore, immune infiltration analysis using ESTIMATE is conducted to evaluate the tumor immune scores of the samples.
The findings reveal a significantly higher expression of MAP4K4 in tumor samples than in adjacent samples. The high-expression group was significantly enriched in tumor-related pathways, such as the PI3K-Akt signaling pathway. In addition, immune infiltration analysis revealed a positive correlation between immune scores and MAP4K4 expression. We also observed that miRNAs, such as miR-192-3p (R = -0.317, p-value 3.111 × 10-9), miR-33b-5p (R= -0.238, p-value 1.166 × 10-5), and miR-582-3p (R = -0.214, p-value 8.430 × 10-5), had potential negative regulatory effects on MAP4K4. Moreover, we identified several transcription factors, ubiquitinated proteins, and interacting proteins that might regulate MAP4K4. The relationship between MAP4K4 and DNA methylation was also identified. Finally, we verified the high expression of MAP4K4 and its effect on promoting cancer.
MAP4K4 might be closely related to gastric cancer\'s progression, invasion, and metastasis. Its high expression negatively impacts the prognosis of gastric cancer patients. This suggests MAP4K4 as an important prognostic factor for gastric cancer and could be regarded as a new potential prognostic detection and therapeutic target.