已发现微RNA参与多种眼部疾病的进展。
■白内障和青光眼经常共存,联合手术是一种常见的治疗方法。本研究旨在分析miR-26a与白内障合并青光眼患者视觉质量的相关性。
■纳入70例白内障合并青光眼患者和70例健康志愿者,接受超声乳化和小梁切除术。根据miR-26a的平均表达将患者分为低和高miR-26a表达组。客观散射指数,斯特雷尔比率,通过光学质量分析系统II分析了调制后的传递函数截止值。miR-26a的变化,客观散射指数,斯特雷尔比率,调制传递函数截止,并对指标之间的相关性进行了分析。通过基因本体论和基因和基因组功能富集的京都百科全书分析miR-26a的下游基因。
■患者和对照组在脂质生物标志物水平和视觉指标方面存在显着差异。miR-26a在患者组中降低。miR-26a低表达组的Strehl比率和调节的传递函数截止值均低于高表达组,而平均视野缺损和客观散射指数均高于高表达组。miR-26a的表达与疾病的严重程度和客观散射指数呈负相关,与Strehl比率和调制传递函数截止值正相关。手术后,miR-26a,斯特雷尔比率,调制传递函数截止值增加,客观散射指数下降。miR-26a的下游基因与多个生物学过程和信号通路有关。
■合并青光眼的白内障患者,miR-26a的表达低于匹配的对照,并且在白内障摘除和小梁切除术后增加。
UNASSIGNED: microRNAs have been found to be involved in the progression of a variety of ocular diseases.
UNASSIGNED: Cataract and glaucoma often coexist, and combined surgery is a common treatment. The aim of this study is to analyse the correlation between miR-26a and visual quality in cataract patients with glaucoma.
UNASSIGNED: Seventy patients with cataract and glaucoma and 70 healthy volunteers were enrolled and received phacoemulsification and trabeculectomy. The patients were divided into low and high miR-26a expression groups according to miR-26a mean expression. The objective scattering index, strehl ratio, and modulated transfer function cut-off were analysed by optical quality analysis system II. The changes of miR-26a, objective scattering index, strehl ratio, modulated transfer function cut-off, and the correlation between the indicators were analysed. The downstream genes of miR-26a were analysed by Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes functional enrichment.
UNASSIGNED: There were significant differences between patients and controls in lipid biomarker levels and visual indicators. miR-26a was decreased in the patient group. Strehl ratio and modulated transfer function cut-off in the miR-26a low-expression group were lower than in high-expression group, while mean defect of the visual field and objective scattering index were higher than in high-expression group. The miR-26a expression was negatively correlated with the severity of disease and objective scattering index, and positively correlated with strehl ratio and modulated transfer function cut-off. After surgery, miR-26a, strehl ratio, and modulated transfer function cut-off were increased, and objective scattering index was decreased. The downstream genes of miR-26a were related to several biological processes and signalling pathways.
UNASSIGNED: In cataract patients with glaucoma, miR-26a expression was lower than matched controls and increased following combined cataract removal and trabeculectomy.