■尽管腹主动脉瘤(AAA)与危及生命的并发症有关,用于诊断目的的可靠生物标志物仍然有限.MicroRNAs(miRNAs)已被提出作为AAA患者的潜在诊断和危险分层标志物。我们旨在评估血清miR-1-3p的水平及其在AAA中的诊断价值。
■这项研究包括200名AAA患者和200名对照。从受试者的医疗记录中收集人口统计学数据和临床信息。根据计算机断层扫描血管造影(CTA)确定AAA形态的个体图像分析。实时定量PCR检测血清miRNA表达水平。生物信息学工具用于鉴定miR-1-3p的靶基因,并进一步丰富其潜在的生物学功能。
■在整体和亚组比较中,AAA组的血清miR-1-3p水平明显低于对照组。它与白细胞呈负相关,CRP,最大动脉瘤直径,area,AAA患者的体积。循环miR-1-3p水平可以显着区分AAA患者和健康个体,曲线下面积(AUC)为0.672(95%CI=0.619-0.724,p<0.001),敏感性为84.5%,特异性为45.5%。血清miR-1-3p与AAA风险降低相关,即使在调整了可能的危险因素后(OR=0.440/单位增加,95%CI=0.301-0.643,p<0.001)。在单变量和多变量逻辑回归分析中,低水平的血清miR-1-3p可以显着提高AAA的风险,OR分别为4.076和4.136(均p<0.001)。进一步的GO富集分析显示miR-1-3p主要参与细胞凋亡过程的负调控,发芽血管生成,血管生成,血管内皮细胞迁移的正向调节,细胞增殖的正向调节,细胞形状的调节,等。
■MiR-1-3p可作为有希望的循环生物标志物用于发展AAA,可能参与多种生物学过程,在AAA发病机制中发挥重要作用。
Although abdominal aortic aneurysm (AAA) is associated with life-threatening complications, there are still limited reliable biomarkers for diagnostic purpose. MicroRNAs (miRNAs) have been proposed as the potential diagnostic and risk stratification markers of AAA patients, and we aim to evaluate the serum level of miR-1-3p and its diagnostic value in AAA.
This study included 200 AAA patients and 200 controls. Demographic data and clinical information were collected from the subjects\' medical records. Individual image analyses of AAA morphology were determined based on computed tomography angiography (CTA). The levels of serum miRNA expression were detected by quantitative real-time PCR. Bioinformatics tools were used to identify the target genes of miR-1-3p and their potential biological functions were further enriched.
Serum miR-1-3p levels in the AAA group were significantly lower when compared with those in the control group in overall and subgroup comparisons. It was negatively related to WBC, CRP, maximal aneurysm diameter, area, and volume in AAA patients. Circulating miR-1-3p levels could significantly discriminate between AAA patients and healthy individuals with an area under the curve (AUC) of 0.672 (95% CI = 0.619-0.724, p < 0.001), a sensitivity of 84.5% and a specificity of 45.5%. Serum miR-1-3p was associated with a reduced risk of AAA even after adjustment for possible risk factors (OR = 0.440 per unit increase, 95% CI = 0.301-0.643, p < 0.001). And low levels of serum miR-1-3p could significantly elevate the risk of AAA in both univariate and multivariate logistic regression analyses with ORs of 4.076 and 4.136, respectively (all p < 0.001). Further GO enrichment analysis revealed that miR-1-3p was mainly involved in negative regulation of apoptotic process, sprouting angiogenesis, angiogenesis, positive regulation of blood vessel endothelial cell migration, positive regulation of cell proliferation, regulation of cell shape, etc.
MiR-1-3p can be used as a promising circulating biomarker in the development of AAA, and it may participate in multiple biological processes to play a crucial role in AAA pathogenesis.