目的:静脉麻醉剂,如依托咪酯和二甲咪酯,在世界范围内广泛传播和滥用,包括在韩国和中国;因此,建立有效和灵敏的分析方法对这些化合物很重要。人的头发作为生物样品具有各种优点,包括广泛的药物检测窗口,与其他典型样本相比,如调查中的尿液和血液。这种交流的目的是开发一种可靠且有用的方法,用于通过超高效液相色谱结合三重四极杆质谱(UPLC-MS/MS)同时检测和定量人发样品中的依托咪酯和二甲咪酯,并将其应用于滥用案件中的真实样本。
方法:用洗涤剂溶液洗涤头发样品,然后是水和丙酮。干燥后,将它们切成大约2毫米的切片,然后用低温研磨机研磨成粉末。将20mg发粉加内标在1mL甲醇中涡旋离心得到上清液层,然后进行分析。
结果:头发样品中依托咪酯和二甲咪酯的校准曲线的测定系数(r2)值均大于0.99,范围为1-500ng/mg和1-500pg/mg,分别。检出限和定量下限分别为0.5和1pg/mg,分别,对于这两种目标化合物。其他测试验证数据均令人满意。在所有的头发样本和烟油中都能检测到依托咪酯和二甲咪酯,被警察查封了.从怀疑的10个样本中获得的依托咪酯和二甲咪酯的浓度为5.48-45.7ng/mg和3.60-377pg/mg,分别。烟油中依托咪酯和二甲咪酯的浓度分别为95.8μg/mg和2.8μg/mg,分别。
结论:在这项研究中,建立了一种简单可靠的人发中依托咪酯和二甲咪酯的分析方法。据我们所知,这是建立同时检测和定量人发中依托咪酯和二甲咪酯的方法的第一份报告,并将其应用于在真实案件中检获的真实样本。
OBJECTIVE: Intravenous narcotic agents, such as etomidate and
metomidate, has been widely spread and abused in the world, including in Korea and China; thus, it is important to establish validated and sensitive analytical method for these compounds. Human hair as a biological sample has various advantages, including a wide detection window of drugs, compared to other typical samples, such as urine and blood in investigation. The purpose of this communication is to develop a reliable and useful method for the simultaneous detection and quantification of etomidate and
metomidate in human hair samples by ultraperformance liquid chromatography combined with triple quadrupole mass spectrometry (UPLC-MS/MS), and to apply it for authentic samples in abuse cases.
METHODS: The hair samples were washed with a detergent solution, followed by with water and acetone. After drying, they were cut into approximately 2 mm sections and then ground to powder by a low-temperature grinder. The 20 mg of hair powder plus internal standard in 1 mL of methanol was vortexed and then centrifuged to obtain the supernatant layer, followed by subjecting to analysis.
RESULTS: The coefficient of determination (r2) values of the calibration curves of etomidate and metomidate in the hair samples were both more than 0.99 in the range of 1-500 ng/mg and 1-500 pg/mg, respectively. The limits of detection and lower limits of quantification were 0.5 and 1 pg/mg, respectively, for the both target compounds. Other tested validation data were all satisfactory. Etomidate and
metomidate could be detected in the all hair samples and cigarette oil, which were seized by the police. The concentrations of etomidate and
metomidate obtained from 10 samples from suspects were 5.48-45.7 ng/mg and 3.60-377 pg/mg, respectively. The concentrations of etomidate and metomidate in the cigarette oil were 95.8 μg/mg and 2.8 μg/mg, respectively.
CONCLUSIONS: In this study, a simple and reliable analytical method for etomidate and
metomidate in the human hair has been established. To the best of our knowledge, this is the first report to establish a method for the simultaneous detection and quantification of etomidate and metomidate in the human hair, and to apply it to authentic samples seized in authentic cases.