■主要的适应性免疫细胞是T和B淋巴细胞,它们在诱导针对犬乳腺肿瘤的免疫应答中起关键作用。研究这些细胞亚群可能会导致对这些恶性肿瘤的更精确诊断。
■CD3+的百分比,将患有恶性乳腺肿瘤的母犬外周血中的CD4和CD8T细胞以及CD21B细胞与健康动物血液中的细胞进行了比较。通过流式细胞术评估外周血白细胞的表型特征。
■健康犬(66.7%)和转移犬(46.1%)之间的CD3淋巴细胞平均百分比存在显着差异,在荷瘤非转移性犬(66.6%)和转移性犬之间。健康犬(40.4%)和转移犬(23.2%)之间的CD4T辅助细胞百分比也存在显着差异,在后者和没有它们的狗之间(35.5%)。在CD21+淋巴细胞亚群的情况下,健康动物(10.9%)和转移动物(20.1%)之间存在显着差异,在后者和没有转移的患者之间(8.5%)。有转移组的CD3+/CD21+比值也有显著差异(3.0),健康组(7.8),和无转移组(8.5)。同样,有转移的动物之间的CD4+/CD8+比率有显著差异(1.4),对照组(2.2)中的母狗,和狗没有转移(1.9)。
■外周血白细胞表型特征是推定的新型生物标志物。这些发现可能对改善乳腺肿瘤诊断程序的未来研究有用。特别是在转移检测中。
UNASSIGNED: The main adaptive immune cells are T and B lymphocytes and they play key roles in the induction of immune responses against canine mammary tumours. Investigating these cell subpopulations may lead to more precise diagnosis of these malignancies.
UNASSIGNED: The percentages of CD3+, CD4+ and CD8+ T cells and of CD21+ B cells in the peripheral blood of bitches with malignant mammary tumours were compared with those in the blood of healthy animals. The phenotypic features of peripheral blood leukocytes were evaluated by flow cytometry.
UNASSIGNED: There was a significant difference in the mean percentages of CD3+ lymphocytes between healthy (66.7%) and metastatic dogs (46.1%), and between tumour-bearing non-metastatic (66.6%) and metastatic dogs. There was also a significant difference in CD4+ T helper cell percentages between healthy dogs (40.4%) and dogs with metastases (23.2%), and between the latter and dogs without them (35.5%). In the case of CD21+ lymphocyte subsets, a significant difference was noted between healthy animals (10.9%) and those with metastases (20.1%), and between the latter and patients without metastases (8.5%). There were also significant differences in CD3+/CD21+ ratios between the group with metastases (3.0), the healthy group (7.8), and the group without metastases (8.5). Similarly, a significant difference was noted in CD4+/CD8+ ratios between animals with metastases (1.4), bitches in the control group (2.2), and dogs without metastases (1.9).
UNASSIGNED: Peripheral blood leukocyte phenotypic characteristics are putative novel biomarkers. These findings may be useful in future studies improving mammary tumour diagnostic procedures, especially in metastasis detection.