背景:介孔二氧化硅纳米颗粒(MSN)被广泛用作递送化疗药物的理想纳米载体。然而,在大多数相关研究中,高抗牙周炎活性和低生物毒性之间的平衡一直难以维持,因为二氧化硅在活生物体中的降解缓慢.
方法:在本研究中,响应性羟基磷灰石(HAP)被掺杂到MSN骨架中,化疗药物盐酸米诺环素(MH)被加载到MSN的孔中,形成带负电荷的药物输送系统。阳离子壳聚糖(COS)是一种抗菌性能高、生物安全性好的生物可降解材料。在这项研究中,通过稳定的电荷相互作用将COS固定在载药颗粒表面,构建了复合给药系统(MH@MSNion@COS)。
结果:体外和细胞实验证明了纳米载体系统的有效降解和药物的同步受控释放。值得注意的是,与单次MH管理相比,这个系统,其中MH和COS共同调节牙周炎相关炎症因子(TNF-α,IL-6,IL-1β,和iNOS),能较好地抑制牙周炎的进展,诱导组织再生,对细胞无明显毒副作用。
结论:该系统为智能,高效,和安全的抗牙周炎药物递送系统。
BACKGROUND: Mesoporous silica nanoparticles (MSN) are widely used as ideal nanovehicles for the delivery of chemotherapeutic drugs. However, the balance between high anti-periodontitis activity and low biotoxicity has been challenging to maintain in most relevant studies owing to the slow degradation of silica in living organisms.
METHODS: In this study, -responsive hydroxyapatite (HAP) was doped into the MSN skeleton, and the chemotherapeutic drug minocycline hydrochloride (MH) was loaded into the pores of MSN, forming a negatively charged drug delivery system. Cationic chitosan (COS) is a biodegradable material with high antibacterial performance and good biosafety. In this study, COS was immobilized on the surface of the drug-loaded particles through stable charge interaction to construct a composite drug delivery system (MH@MSNion@COS).
RESULTS: In vitro and cellular experiments demonstrated effective degradation of the nanocarrier system and synchronized controlled release of the drug. Notably, compared with single MH administration, this system, in which MH and COS jointly regulated the expression levels of periodontitis- associated inflammatory factors (TNF-α, IL-6, IL-1β, and iNOS), better inhibited the progress of periodontitis and induced tissue regeneration without showing significant toxic side effects in cells.
CONCLUSIONS: This system provides a promising strategy for the design of intelligent, efficient, and safe anti-periodontitis drug delivery systems.