鸽副粘病毒1型(PPMV-1)属于禽副粘病毒1型病毒群,会引起震颤,斜颈,家鸽和野鸽的呼吸体征。PPMV-1的M蛋白是一种多功能结构蛋白。它不仅有助于装配,萌芽,和病毒的定位,但也会抑制宿主的免疫反应,促进病毒在宿主中的复制。在这项研究中,GST下拉法用于筛选与PPMV-1M蛋白相互作用的宿主蛋白,然后使用质谱(MS)分析筛选的宿主蛋白。对差异表达基因的富集分析表明,筛选的77种蛋白质与基因本体论类别高度相关:蛋白质合成,新陈代谢,和细胞信号传导途径。我们选择NIMA相关激酶7(NEK7)作为候选蛋白进行共定位分析和共免疫沉淀验证。结果表明,PPMV-1M蛋白与宿主细胞的NEK7相互作用。PPMV-1M蛋白的相互作用研究将有助于阐明其在病毒复制过程中的功能,并为研究PPMV-1的致病机制提供重要的理论基础。
Pigeon paramyxovirus type 1 (PPMV-1) belongs to the avian paramyxovirus type 1 group of viruses, which can cause tremors, torticollis, and respiratory signs in domestic and wild pigeons. The M protein of PPMV-1 is a multifunctional structural protein. It not only helps in the assembly, budding, and positioning of the virus but also inhibits the host\'s immune response and promotes replication of the virus in the host. In this study, the GST pull-down method was used to screen host proteins that interact with PPMV-1 M protein, and then mass spectrometry (MS) was used to analyse the screened host proteins. Enrichment analysis of the differentially expressed genes showed that the 77 screened proteins were highly associated with the gene ontology categories: protein synthesis, metabolism, and cell signalling pathway transduction. We selected NIMA-related kinase 7 (NEK7) as the candidate protein for co-localization analysis and co-immunoprecipitation verification. The results revealed that PPMV-1 M protein interacts with NEK7 of the host cell. This interactome study of PPMV-1 M protein will serve to clarify its function during viral replication and will provide a crucial theoretical basis for studying the pathogenic mechanism of PPMV-1.