背景:了解采血和交配之间的关系对于有效管理最适应的媒介昆虫很重要,埃及伊蚊(Linnaeus)。尽管广泛的研究已经调查了伊蚊的行为方面,如血液喂养,交配,和他们的关系,一些知识差距仍然存在。因此,本研究是为了确定未喂食成功交配的可能性,被激怒了,部分妊娠至完全妊娠(在用完全发育的卵喂养血液后长达5天)雌性Ae。埃及伊蚊和产卵。
方法:以三种不同方式允许性成熟的成年埃及伊蚊交配。在对照1中,雌性在服用血餐之前被允许交配,在对照2中,雌性根本不允许交配。在实验中,女性分为六类,viz.D-0到D-5.在D-0中,允许雌性在血后立即交配,在D-1中,雌性在血液喂养的第一天被允许交配,同样,D-2,D-3,D-4和D-5的雌性在2日被允许交配,3rd,第4天和第5天的血液喂养。将诱蚊产卵器在第4天均匀地保存在笼子D-0至D-3的血液喂养1小时,然后将其取出,对于笼子D-4和D-5,将诱蚊产卵器在交配后立即进行血液喂养1小时。计数卵的总数和孵化的总数。在随后的日子里,整个实验用不同的蚊子群重复了两次,三个实验的平均值用于绘制具有5%误差条的Excel条,并用于统计分析。
结果:发现产卵前的交配足以产生1581个卵(与对照组相比为70%)并使1369个卵受精(与产卵总数相比为85%),远高于未交配雌性产下的676个未孵化(未受精)卵(30%)。虽然交配不是产卵所必需的,我们的研究表明,即使短暂暴露于精液和精液,也会大大提高产卵和孵化效率,即使交配发生在产卵前。然而,那些在采血前交配的雌性和在采血后交配的雌性产生了2266和2128个卵,孵化率分别为96.78%和95.54%,分别。因此,精液在雌性中的保留时间似乎会影响产卵的数量和孵化的卵的数量。
结论:一般来说,交配是可能的Ae。埃及伊蚊甚至在产卵前几分钟,足以产生更多的可行卵。
BACKGROUND: Understanding the relationship between blood-feeding and
mating is important in effectively managing the most well-adapted vector insect, Aedes aegypti (Linnaeus). Although extensive studies have investigated the behavioural aspects of Aedes such as blood-feeding,
mating, and their relationship, several knowledge gaps still exist. Therefore, the present study was undertaken to determine the possibility of successful
mating by unfed, engorged, and partially to fully gravid (up to 5 days after blood-feeding with fully developed eggs) female Ae. aegypti mosquitoes and production of viable eggs.
METHODS: Mating of sexually mature adult Aedes aegypti was allowed in three different ways. In control 1, the females were allowed to mate before taking blood meal, and in control 2, the females were not at all allowed to mate. In the experiment, the females were separated into six categories, viz. D-0 to D-5. In D-0, the females were allowed to mate immediately after the bloodmeal and, in D-1, the females were allowed to mate on the first day of blood feeding, likewise, the females of D-2, D-3, D-4 and D-5 were allowed to mate on 2nd, 3rd, 4th and 5th day of blood feeding. Ovitrap was uniformly kept on the 4th day of blood feeding for the cages D-0 to D-3 for 1 h and then removed and for the cages D-4, and D-5, the ovitrap was kept on 4th and 5th day of blood feeding for 1h immediately after mating. The total number of eggs and the total number of hatching were counted. In the subsequent days, the entire experiment was replicated two times with different cohorts of mosquitoes, and the mean value of three experiments was used to draw Excel bars with 5% error bars and also for the statistical analysis.
RESULTS: It was found that mating just before oviposition was sufficient to produce 1581 eggs (70% compared with control) and fertilize 1369 eggs (85% compared with total eggs laid), which is far higher than the 676 non-hatching (unfertilized) eggs (30%) laid by unmated females. Although mating is not essential for producing eggs, our study shows that even brief exposure to the semen and seminal fluids greatly enhances the oviposition and hatching efficiency, even if the mating occurs just before oviposition. However, those females
mating before blood-feeding and those
mating after blood-feeding produced 2266 and 2128 eggs, with hatching rates of 96.78% and 95.54%, respectively. Hence, the retention time of seminal fluid in the female seems to influence the number of eggs laid and the number of eggs hatched.
CONCLUSIONS: In general, mating is possible in Ae. aegypti even minutes before oviposition and is sufficient to produce a greater number of viable eggs.