背景:在欧洲传统植物疗法中,来自不同草药植物的提取物用于预防和治疗简单的尿路感染,并用于冲洗出肾脏病。除了轻微的利尿作用增加尿流,也刺激Tamm-Horsfall蛋白(syn.THP,肾脏远端的尿调蛋白)可以解释降低的肾脏砾石和对尿路致病性大肠杆菌的抗毒活性。
目的:评价木贼提取物的THP诱导活性,浮子,Paraguariensis,Juniperuscommunis,荨麻,和蒲公英通过定量口服给人体后的尿液样品中的THP。
方法:对健康志愿者(每个干预组n=10)进行7天p.o.以及通过验证的ELISA对THP含量的第3、6和8天。使用UPEC菌株NU14通过流式细胞术监测尿液样品对人T24膀胱细胞的抗粘附活性。
结果:来自E.arvense的水提物,通过特定的LC-MS方法充分表征,在7天p.o.施用期间,尿液样品中诱导的THP浓度显着达到300%,与未经处理的对照有关。对THP浓度升高的个体和汇集的尿液样品的离体研究显示出与针对UPECNU14对T24细胞的抗粘附作用的良好相关性。木贼处理的志愿者的尿液样品对UPECNU14的增殖和生物膜形成没有影响。尿液样品中的二氧化硅排泄与各自的THP水平无关。监测尿液样品中的电解质含量表明木贼提取物干预的利尿作用。通过LC-MS和LC-UV对木贼提取物进行详细的植物化学分析,揭示了分析方案,通过MS评估从提取物中鉴定出>80种化合物,通过UV检测鉴定出18种化合物。该协议将为木贼提取物的未来质量控制提供有价值的工具。
结论:口服7天后,来自E.arvense的水提取物显着刺激尿液样品中的THP分泌,并抑制UPEC和膀胱宿主细胞之间的相互作用。这可以解释这种草药用于尿路感染和肾脏砾石的治疗用途。通过LC-MS和LC-UV对木贼提取物进行详细的植物化学分析,揭示了分析方案,这鉴定了>82%的所有洗脱化合物。该协议将为木贼提取物的未来质量控制提供有价值的工具。
BACKGROUND: Within European traditional phytotherapy, extracts from different herbal plants are used for prevention and therapy of uncomplicated urinary tract infections and for flushing out of kidney grits. Besides increased urine flow by slight diuretic effects, also stimulation of Tamm-Horsfall protein (syn. THP, uromodulin) in the distal part of the kidney could explain reduced kidney gravel and anti-virulent activity against uropathogenic E. coli.
OBJECTIVE: Evaluation of THP-inducing activity of extracts from Equisetum arvense, Levisticum officinalis, Ilex paraguariensis, Juniperus communis, Urtica dioica, and Taraxacum officinale by quantification of THP in urine samples after oral application to humans.
METHODS: 7 days p.o. application of the test intervention to healthy volunteers (n = 10 per intervention group) and analysis of urine samples at day 1 (untreated control values), and days 3, 6 and 8 on THP content by validated ELISA. Antiadhesive activity of urine samples was monitored by flow cytometry using UPEC strain NU14 against human T24 bladder cells.
RESULTS: An aqueous extract from E. arvense, fully characterized by a specific LC-MS method, induced THP concentration in urine samples significantly during a 7-day p.o. application up to 300%, related to the untreated controls. Ex vivo investigation of the individual and pooled urine samples with elevated THP concentrations showed good correlation to antiadhesive effects against UPEC NU14 to T24 cells. Urine samples of the Equisetum treated volunteers had no effect on the proliferation and on biofilm formation of UPEC NU14. Silica excretion in the urine samples had no correlation to the respective THP levels. Monitoring of electrolyte content in the urine samples indicat ed diuretic effects of the intervention with Equisetum extract. Detailed phytochemical analysis of the Equisetum extract by LC-MS and LC-UV revealed an analytical protocol, which identified > 80 compounds from the extract by MS evaluations and 18 compounds by UV detection. This protocol will provide a valuable tool for future quality control of Equisetum extract.
CONCLUSIONS: Aqueous extract from E. arvense significantly stimulates THP secretion in urine samples after 7 days of oral intake and inhibits the interplay between UPEC and bladder host cells. This could explain the therapeutic use of this herbal material for urinary tract infections and kidney gravel. Detailed phytochemical analysis of the Equisetum extract by LC-MS and LC-UV revealed an analytical protocol, which identified > 82% of all eluted compounds. This protocol will provide a valuable tool for future quality control of Equisetum extract.