革兰氏阴性,有氧,杆状,不活动,黄色色素细菌,KMM9835T,是从日本海阿穆尔湾海岸获得的沉积物样本中分离出来的,俄罗斯。基于16SrRNA基因和全基因组序列的系统发育分析将Mariniflexile属中的新菌株KMM9835T定位为一个单独的品系,与MariniflexilesosokkakenseRSSK-9T和MariniflexilefucleaskkakenseSW5T共享96.6%和96.2%的最高16SrRNA基因序列相似性,分别,与其他公认的Mariniflexile物种的相似性值<96%。菌株KMM9835T和M.soesokkakenseKCTC32427T之间的平均核苷酸同一性和数字DNA-DNA杂交值,MariniflexilegromoviiKCTC12570T,M.fucanivoransDSM18792T,和M.maritimumM5A1MT为83.0%,82.5%,83.4%,78.3%和30.7%,29.6%,29.5%,和24.4%,分别。菌株KMM9835T的基因组DNAGC含量为32.5mol%。主要的甲萘醌是MK-6,主要的脂肪酸是异C15:0,异C15:1ω10c,C15:0菌株KMM9835T的极性脂质由磷脂酰乙醇胺组成,两种身份不明的氨基脂类,一种身份不明的磷脂,和六种身份不明的脂质。全基因组分析表明,KMM9835T基因组编码753个单例。注释的单例通常与转运蛋白系统(SusC)相关,转录调节因子(AraC,LytTR,LacI),和酶(糖基化酶)。KMM9835T基因组高度富含CAZyme编码基因,比例达到7.3%。此外,KMM9835T基因组的特征是CAZyme基因家族(GH43,GH28,PL1,PL10,CE8和CE12)的丰度很高,表明它有可能分解果胶.这可能是适应策略的一部分,可促进在海岸线和淡水来源附近的水生环境中微生物消耗植物聚合物基质。基于系统发育和表型表征的组合,海洋沉积物菌株KMM9835T(=KCTC92792T)代表了Mariniflexile属的一种新物种,名称为Mariniflexilelitoralesp。11月。是提议的。
A Gram-negative, aerobic, rod-shaped, non-motile, yellow-pigmented bacterium, KMM 9835T, was isolated from the sediment sample obtained from the Amur Bay of the Sea of Japan seashore, Russia. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences positioned the novel strain KMM 9835T in the genus Mariniflexile as a separate line sharing the highest 16S rRNA gene sequence similarities of 96.6% and 96.2% with Mariniflexile soesokkakense RSSK-9T and Mariniflexile fucanivorans SW5T, respectively, and similarity values of <96% to other recognized Mariniflexile species. The average nucleotide identity and digital DNA-DNA hybridization values between strain KMM 9835T and M. soesokkakense KCTC 32427T, Mariniflexile gromovii KCTC 12570T, M. fucanivorans DSM 18792T, and M. maritimum M5A1MT were 83.0%, 82.5%, 83.4%, and 78.3% and 30.7%, 29.6%, 29.5%, and 24.4%, respectively. The genomic DNA GC content of strain KMM 9835T was 32.5 mol%. The dominant menaquinone was MK-6, and the major fatty acids were iso-C15:0, iso-C15:1ω10c, and C15:0. The polar lipids of strain KMM 9835T consisted of phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid, and six unidentified lipids. A pan-genome analysis showed that the KMM 9835T genome encoded 753 singletons. The annotated singletons were more often related to transport protein systems (SusC), transcriptional regulators (AraC, LytTR, LacI), and enzymes (glycosylases). The KMM 9835T genome was highly enriched in CAZyme-encoding genes, the proportion of which reached 7.3%. Moreover, the KMM 9835T genome was characterized by a high abundance of CAZyme gene families (GH43, GH28, PL1, PL10, CE8, and CE12), indicating its potential to catabolize pectin. This may represent part of an adaptation strategy facilitating microbial consumption of plant polymeric substrates in aquatic environments near shorelines and freshwater sources. Based on the combination of phylogenetic and phenotypic characterization, the marine sediment strain KMM 9835T (=KCTC 92792T) represents a novel species of the genus Mariniflexile, for which the name Mariniflexile litorale sp. nov. is proposed.