■了解肠道通透性对于阐明胃肠道健康和病理至关重要。穿过肠屏障的分子的大小和性质为各种急性和慢性疾病提供了重要的见解。以及一些条件的演变。本研究旨在评估谷蛋白免疫原性肽(u-GIP)的尿排泄动力学,尿液中可检测到的一类独特的膳食肽,在受控饮食条件下的志愿者中。此评估应与乳果糖等已建立的探针进行比较,一种不可消化的二糖,表明细胞旁通透性,和甘露醇,反映跨细胞通透性。
■15名参与者同时摄入标准剂量的谷蛋白(10克),乳果糖(10g),和甘露醇(1g)在禁食条件下摄入前至少8小时和摄入后6小时期间。在指定的时间间隔内收集尿样。对排泄模式进行了分析,并评估乳果糖与甘露醇比率(LMR)与u-GIP参数之间的相关性。
■在摄入后的前12小时内检测到大多数u-GIP。对两个样品收集范围的累积排泄变异性的分析表明,乳果糖和u-GIP表现出相似的起效和排泄动力学。尽管GIP比乳果糖或甘露醇更早达到其最大峰值。此外,在最长的尿液收集间隔内,LMR和u-GIP参数之间观察到中度相关性,表明渗透途径之间潜在的共同特征。这些发现表明,延长尿液收集超过6小时可能会增强数据的可靠性。
■这项研究揭示了u-GIP与乳果糖和甘露醇相比的时间动力学,建立了评估肠道通透性的探针。u-GIP和乳果糖排泄模式之间的相似性与预期的细胞旁通透性途径一致。检测尿液中抗原性食物蛋白质片段的能力为研究蛋白质代谢和监测与消化系统和肠道系统相关的病理开辟了新途径。
UNASSIGNED: Understanding intestinal permeability is paramount for elucidating gastrointestinal health and pathology. The size and nature of the molecule traversing the intestinal barrier offer crucial insights into various acute and chronic diseases, as well as the evolution of some conditions. This study aims to assess the urinary excretion kinetics of gluten immunogenic peptides (u-GIP), a unique class of dietary peptides detectable in urine, in volunteers under controlled dietary conditions. This evaluation should be compared to established probes like lactulose, a non-digestible disaccharide indicative of paracellular permeability, and
mannitol, reflecting transcellular permeability.
UNASSIGNED: Fifteen participants underwent simultaneous ingestion of standardized doses of gluten (10 g), lactulose (10 g), and
mannitol (1 g) under fasting conditions for at least 8 hours pre-ingestion and during 6 hours post-ingestion period. Urine samples were collected over specified time intervals. Excretion patterns were analyzed, and correlations between the lactulose-to-
mannitol ratio (LMR) and u-GIP parameters were assessed.
UNASSIGNED: The majority of u-GIP were detected within the first 12 hours post-ingestion. Analysis of the variability in cumulative excretion across two sample collection ranges demonstrated that lactulose and u-GIP exhibited similar onset and excretion dynamics, although GIP reached its maximum peak earlier than either lactulose or
mannitol. Additionally, a moderate correlation was observed between the LMR and u-GIP parameters within the longest urine collection interval, indicating potential shared characteristics among permeability pathways. These findings suggest that extending urine collection beyond 6 hours may enhance data reliability.
UNASSIGNED: This study sheds light on the temporal dynamics of u-GIP in comparison to lactulose and
mannitol, established probes for assessing intestinal permeability. The resemblance between u-GIP and lactulose excretion patterns aligns with the anticipated paracellular permeability pathway. The capacity to detect antigenic food protein fragments in urine opens novel avenues for studying protein metabolism and monitoring pathologies related to the digestive and intestinal systems.