背景:铬(Cr)毒性显着威胁全球农业生态系统,对植物生长和发育产生不利影响,降低作物生产力。海藻糖,一种非还原糖已被确定为由非生物胁迫如干旱引起的毒性效应的缓解剂,盐度,和重金属。这项研究的主要目的是研究外源施用海藻糖对暴露于Cr胁迫的玉米植物的影响。
结果:两个玉米品种,对FH-1046和FH-1453进行两种不同的Cr浓度(0.3mM,和0.5mM)。结果表明,与对照组相比,在Cr诱导的胁迫条件下,两个玉米品种的生长和生化参数存在显着差异。两种玉米品种均以30mM的浓度叶面施用海藻糖,导致Cr应力的有害影响显著降低。值得注意的是,Cr(0.5mM)应力对枝条长度的影响大于0.3mM的Cr应力。Cr胁迫(0.5mM)使FH-1046和FH-1453中的芽长度显着降低了12.4%,而海藻糖使FH-1046和FH-1453中的芽长度分别增加了30.19%和4.75%。Cr胁迫显著制约生长和生化过程,而海藻糖通过减少Cr的吸收和最大程度地减少Cr引起的氧化应激,显着改善了植物的生长。这种氧化应激的减少由脯氨酸的产生减少证明,SOD,POD,MDA,H2O2,过氧化氢酶,和APX。海藻糖还能增强Cr胁迫下的光合活性,如叶绿素a值增加所示,B,和类胡萝卜素。此外,通过蛋白质和碳水化合物含量的增加和Cr摄取的减少证明了海藻糖的改善潜力。
结论:该研究表明,海藻糖的施用大大改善了两个玉米品种的生长并增强了光合活性。海藻糖(30mM)显着增加植物生物量,即使在0.5mM时,ROS的产生也会减少,对Cr胁迫的抵抗力也会增强。
BACKGROUND: Chromium (Cr) toxicity significantly threatens agricultural ecosystems worldwide, adversely affecting plant growth and development and reducing crop productivity. Trehalose, a non-reducing sugar has been identified as a mitigator of toxic effects induced by abiotic stressors such as drought, salinity, and heavy metals. The primary objective of this study was to investigate the influence of exogenously applied trehalose on
maize plants exposed to Cr stress.
RESULTS: Two
maize varieties, FH-1046 and FH-1453, were subjected to two different Cr concentrations (0.3 mM, and 0.5 mM). The results revealed significant variations in growth and biochemical parameters for both
maize varieties under Cr-induced stress conditions as compared to the control group. Foliar application of trehalose at a concentration of 30 mM was administered to both
maize varieties, leading to a noteworthy reduction in the detrimental effects of Cr stress. Notably, the Cr (0.5 mM) stress more adversely affected the shoot length more than 0.3mM of Cr stress. Cr stress (0.5 mM) significantly reduced the shoot length by 12.4% in FH-1046 and 24.5% in FH-1453 while Trehalose increased shoot length by 30.19% and 4.75% in FH-1046 and FH-1453 respectively. Cr stress significantly constrained growth and biochemical processes, whereas trehalose notably improved plant growth by reducing Cr uptake and minimizing oxidative stress caused by Cr. This reduction in oxidative stress was evidenced by decreased production of proline, SOD, POD, MDA, H2O2, catalase, and APX. Trehalose also enhanced photosynthetic activities under Cr stress, as indicated by increased values of chlorophyll a, b, and carotenoids. Furthermore, the ameliorative potential of trehalose was demonstrated by increased contents of proteins and carbohydrates and a decrease in Cr uptake.
CONCLUSIONS: The study demonstrates that trehalose application substantially improved growth and enhanced photosynthetic activities in both
maize varieties. Trehalose (30 mM) significantly increased the plant biomass, reduced ROS production and enhanced resilience to Cr stress even at 0.5 mM.