类木质素是在许多疾病的治疗中发挥强大的抗氧化和抗炎作用的活性成分。为了提高中药废弃物的资源化利用效率,本研究以厚朴和E.H.Wilson残渣(MOR)废物生物质为原料,和一系列低共熔溶剂(ChUre,ChAce,ChPro,ChCit,ChOxa,ChMal,ChLac,Chlev,选择ChGly和ChEG)来评估从MORs中提取木脂素的效率。结果表明,木脂素提取的最佳条件为液固比40.50mL/g,HBD-HBA比率为2.06,水百分比为29.3%,提取物温度为337.65K,和107分钟的时间。在这些条件下,木脂素的最大含量为39.18mg/g。此外,通过数学模型研究了萃取过程的动力学。在我们的抗氧化活性研究中,木脂素提取物的高抗氧化活性在清除四种不同类型的自由基(DPPH,·OH,ABTS,和超氧化物阴离子)。在3mg/mL的浓度下,木脂素提取物的总抗氧化能力为1.795U/mL,等于0.12mg/mL的Vc溶液。此外,抗菌活性研究发现,木脂素提取物对六种被测病原体具有良好的抗菌作用。其中,金黄色葡萄球菌的抗菌活性最强。最终,木脂素提取物与DES的生物活性和物理化学性质的相关性使用热图描述,随着体外降血糖的评估,体外降血脂,免疫调节,木脂素提取物的抗炎活性。这些发现可以为低共熔溶剂从废弃生物质中提取高价值成分提供理论依据。以及强调其在天然产品开发和利用中的具体意义。
Lignanoids are an active ingredient exerting powerful antioxidant and anti-inflammatory effects in the treatment of many diseases. In order to improve the efficiency of the resource utilization of traditional Chinese medicine waste, Magnolia officinalis Rehder & E.H.Wilson residue (MOR) waste biomass was used as raw material in this study, and a series of deep eutectic solvents (ChUre, ChAce, ChPro, ChCit, ChOxa, ChMal, ChLac, ChLev, ChGly and ChEG) were selected to evaluate the extraction efficiency of lignanoids from MORs. The results showed that the best conditions for lignanoid extraction were a liquid-solid ratio of 40.50 mL/g, an HBD-HBA ratio of 2.06, a water percentage of 29.3%, an extract temperature of 337.65 K, and a time of 107 min. Under these conditions, the maximum lignanoid amount was 39.18 mg/g. In addition, the kinetics of the extraction process were investigated by mathematic modeling. In our antioxidant activity study, high antioxidant activity of the lignanoid extract was shown in scavenging four different types of free radicals (DPPH, ·OH, ABTS, and superoxide anions). At a concentration of 3 mg/mL, the total antioxidant capacity of the lignanoid extract was 1.795 U/mL, which was equal to 0.12 mg/mL of Vc solution. Furthermore, the antibacterial activity study found that the lignanoid extract exhibited good antibacterial effects against six tested pathogens. Among them, Staphylococcus aureus exerted the strongest antibacterial activity. Eventually, the correlation of the lignanoid extract with the biological activity and physicochemical properties of DESs is described using a heatmap, along with the evaluation of the in vitro hypoglycemic, in vitro hypolipidemic, immunomodulatory, and anti-inflammatory activity of the lignanoid extract. These findings can provide a theoretical foundation for the extraction of high-value components from waste biomass by deep eutectic solvents, as well as highlighting its specific significance in natural product development and utilization.