背景:奶牛的脂肪肝是一种常见的代谢疾病,由肝细胞中的甘油三酯(TG)积聚定义。脂肪肝的临床诊断通常通过肝活检来完成,由于缺乏更有效的诊断方法,在乳制品行业造成了相当大的经济损失。因此,本研究旨在探讨血液生物标志物在奶牛脂肪肝诊断和预警中的潜在应用价值。
结果:在分娩后28天内随机选择24头泌乳母牛作为实验动物,并将其分为健康母牛(进行肝活检,n=12)和患有脂肪肝的奶牛(肝活检测试,n=12)。采用电感耦合等离子体质谱法(ICP-MS)测定两组奶牛血清中的宏观元素和微量元素。与健康奶牛(C)相比,钙(Ca)的浓度,钾(K),镁(Mg),锶(Sr),硒(Se),锰(Mn),脂肪肝奶牛(F)中硼(B)和钼(Mo)较低,铜(Cu)较高。同时,观察到的宏观元素和微量元素的差异与交货时间有关,C和F之间最大的主要差异发生在分娩后7天。采用多变量分析方法检验了9种血清宏观元素之间的相关性,微量元素和脂肪肝。基于可变重要性投影和接收者工作特性(ROC)曲线分析,矿物质Ca,Se,K,筛选出B和Mo作为产后奶牛脂肪肝的最佳诊断指标。
结论:我们的数据表明血清钙水平,K,Mg,Se,B,Mo,Mn,和Sr在F中低于C中。最适合早期识别奶牛脂肪肝的时期是分娩后7天,Ca,Se,K,B和Mo是产后奶牛脂肪肝的最佳诊断指标。
BACKGROUND: Fatty liver in dairy cows is a common metabolic disease defined by triglyceride (TG) buildup in the hepatocyte. Clinical diagnosis of fatty liver is usually done by liver biopsy, causing considerable economic losses in the dairy industry owing to the lack of more effective diagnostic methods. Therefore, this study aimed to investigate the potential utility of blood biomarkers for the diagnosis and early warning of fatty liver in dairy cows.
RESULTS: A total of twenty-four lactating cows within 28 days after parturition were randomly selected as experimental animals and divided into healthy cows (liver biopsy tested, n = 12) and cows with fatty liver (liver biopsy tested, n = 12). Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the
macroelements and microelements in the serum of two groups of cows. Compared to healthy cows (C), concentrations of calcium (Ca), potassium (K), magnesium (Mg), strontium (Sr), selenium (Se), manganese (Mn), boron (B) and molybdenum (Mo) were lower and copper (Cu) was higher in fatty liver cows (F). Meanwhile, the observed differences in
macroelements and microelements were related to delivery time, with the greatest major disparity between C and F occurring 7 days after delivery. Multivariable analysis was used to test the correlation between nine serum
macroelements, microelements and fatty liver. Based on variable importance projection and receiver operating characteristic (ROC) curve analysis, minerals Ca, Se, K, B and Mo were screened as the best diagnostic indicators of fatty liver in postpartum cows.
CONCLUSIONS: Our data suggested that serum levels of Ca, K, Mg, Se, B, Mo, Mn, and Sr were lower in F than in C. The most suitable period for an early-warning identification of fatty liver in cows was 7 days after delivery, and Ca, Se, K, B and Mo were the best diagnostic indicators of fatty liver in postpartum cows.