■断奶压力引起的肠功能障碍在母乳喂养期间很常见。二十二碳六烯酸(DHA)以促进视觉和大脑发育而闻名,但其对早期肠道发育的影响尚不清楚。这项研究调查了哺乳期母亲补充DHA对断奶后代小鼠肠道葡萄糖吸收和肠道菌群的影响。
■大坝补充了车辆(对照),150mg/(kg体重·天)DHA(L-DHA),或450mg/(kg体重·天)DHA(H-DHA)在整个哺乳期通过口服给药。断奶后,幼崽被随机分为三组进行运动分析,微生物和蛋白质组学分析,生化分析,4-脱氧-4-氟-D-葡萄糖(4-FDG)吸收试验,以及葡萄糖转运相关蛋白和mTOR信号传导成分的基因表达定量。
■与对照组相比,H-DHA组表现出增强的抓地力和延长的游泳时间。此外,空肠和回肠绒毛高度显著增加,H-DHA组空肠绒毛表面积扩大。微生物分析显示,母体DHA的摄入增加了有益肠道细菌的丰度,并促进了与碳水化合物和能量代谢相关的代谢途径。蛋白质组学研究表明,在H-DHA组中,营养转运蛋白的丰度增加,并且涉及吸收和消化的途径富集。该组还显示空肠和回肠中葡萄糖浓度较高,以及肝脏和肌肉中糖原水平升高,与对照组相比,肠道内容物和粪便中的葡萄糖水平较低。4-FDG吸收试验显示H-DHA组口服4-FDG灌胃后更有效的吸收。此外,葡萄糖转运相关蛋白的表达,与对照组相比,H-DHA组的GLUT2和SGLT1以及mTOR通路的激活增强。相对于H-DHA组,L-DHA组在这些方面也显示出相似但不太明显的改善。
■我们的研究结果表明,母亲在哺乳期补充DHA可以改善运动表现,通过增加葡萄糖转运蛋白的表达来增强肠道葡萄糖的吸收,并有益地改变断奶后代小鼠肠道微生物组的结构。
UNASSIGNED: Intestinal dysfunction induced by weaning stress is common during breastfeeding period. Docosahexaenoic acid (DHA) is well known for promoting visual and brain development, but its effects on early intestinal development remain unknown. This study investigated the impact of maternal DHA supplementation during lactation on intestinal glucose absorption and gut microbiota in weaning offspring mice.
UNASSIGNED: Dams were supplemented with vehicle (control), 150 mg/(kg body weight · day) DHA (L-DHA), or 450 mg/(kg body weight · day) DHA (H-DHA) throughout lactation by oral administration. After weaning, pups were randomly divided into three groups for athletic analysis, microbial and proteomic analysis, biochemical analysis, 4-deoxy-4-fluoro-D-glucose (4-FDG) absorption test, and gene expression quantitation of glucose transport-associated proteins and mTOR signaling components.
UNASSIGNED: The H-DHA group exhibited enhanced grip strength and prolonged swimming duration compared to the control group. Additionally, there were significant increases in jejunal and ileal villus height, and expanded surface area of jejunal villi in the H-DHA group. Microbial analyses revealed that maternal DHA intake increased the abundance of beneficial gut bacteria and promoted metabolic pathways linked to carbohydrate and energy metabolism. Proteomic studies indicated an increased abundance of nutrient transport proteins and enrichment of pathways involved in absorption and digestion in the H-DHA group. This group also showed higher concentrations of glucose in the jejunum and ileum, as well as elevated glycogen levels in the liver and muscles, in contrast to lower glucose levels in the intestinal contents and feces compared to the control group. The 4-FDG absorption test showed more efficient absorption after oral 4-FDG gavage in the H-DHA group. Moreover, the expressions of glucose transport-associated proteins, GLUT2 and SGLT1, and the activation of mTOR pathway were enhanced in the H-DHA group compared to the control group. The L-DHA group also showed similar but less pronounced improvements in these aspects relative to the H-DHA group.
UNASSIGNED: Our findings suggested that maternal DHA supplementation during lactation improves the exercise performance, enhances the intestinal glucose absorption by increasing the expressions of glucose transporters, and beneficially alters the structure of gut microbiome in weaning offspring mice.