淋巴毒素β受体(LTBR)是一种阳性的T细胞增殖调控基因。它与肿瘤免疫微环境密切相关。然而,其在癌症和免疫治疗中的作用尚不清楚.首先,分析LTBR的表达水平和预后价值。其次,LTBR在临床分期中的表达,免疫亚型,并对分子亚型进行了分析。LTBR与免疫调节基因的相关性,免疫检查点基因,然后分析RNA修饰基因。LTBR与免疫细胞的相关性,分数,癌症相关的功能状态,肿瘤干性指数,错配修复(MMR)基因,和DNA甲基转移酶也被分析。此外,我们分析了LTBR在DNA甲基化中的作用,突变状态,肿瘤突变负荷(TMB),和微卫星不稳定性(MSI)。基因本体论(GO),京都基因和基因组百科全书(KEGG),和基因集富集分析(GSEA)用于探索LTBR在泛癌症中的作用。最后,分析了与LTBR相关的药物.使用定量实时PCR和Western印迹确认LTBR的表达。LTBR在大多数癌症中显著过表达,并且与低患者存活率相关。此外,LTBR表达与免疫细胞密切相关,得分,癌症相关的功能状态,肿瘤干性指数,MMR基因,DNA甲基转移酶,DNA甲基化,突变状态,TMB,和MSI。富集分析显示LTBR与细胞凋亡有关,坏死,和免疫相关途径。最后,确定了多种靶向LTBR的药物。LTBR在几种肿瘤中过表达并且与不良预后相关。它与免疫相关基因和免疫细胞浸润有关。
Lymphotoxin beta receptor (LTBR) is a positive T cell proliferation regulator gene. It is closely associated with the tumor immune microenvironment. However, its role in cancer and immunotherapy is unclear. Firstly, the expression level and prognostic value of LTBR were analyzed. Secondly, the expression of LTBR in clinical stages, immune subtypes, and molecular subtypes was analyzed. The correlation between LTBR and immune regulatory genes, immune checkpoint genes, and RNA modification genes was then analyzed. Correlations between LTBR and immune cells, scores, cancer-related functional status, tumor stemness index, mismatch repair (MMR) genes, and DNA methyltransferase were also analyzed. In addition, we analyzed the role of LTBR in DNA methylation, mutational status, tumor mutation burden (TMB), and microsatellite instability (MSI). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were used to explore the role of LTBR in pan-cancer. Finally, the drugs associated with LTBR were analyzed. The expression of LTBR was confirmed using quantitative real-time PCR and Western blot. LTBR is significantly overexpressed in most cancers and is associated with low patient survival. In addition, LTBR expression was strongly correlated with immune cells, score, cancer-related functional status, tumor stemness index, MMR genes, DNA methyltransferase, DNA methylation, mutational status, TMB, and MSI. Enrichment analysis revealed that LTBR was associated with apoptosis, necroptosis, and immune-related pathways. Finally, multiple drugs targeting LTBR were identified. LTBR is overexpressed in several tumors and is associated with a poor prognosis. It is related to immune-related genes and immune cell infiltration.