UNASSIGNED:经皮给药提供了一种用于牛皮癣治疗的穿透皮肤的理想替代方法,凭借其抑制免疫细胞过度活化和炎症的能力,同时减轻全身给药的有害影响。淋巴结(LNs),作为淋巴和获得性免疫系统的重要器官,是药物归巢抑制免疫细胞的合适部位。
未经评估:在这种情况下,我们开发了一种微针(MN)贴片,将纳米药物局部递送至LNs,以改善抗银屑病治疗.在这项研究中,合成了携带甲氨蝶呤(HM)的人血清白蛋白纳米颗粒,并将其加载到基于透明质酸(HA)的微针(HM/MN)中。
未经ASSIGNED:该贴片显示出极好的刺穿皮肤的能力,这增强了药物输送。在牛皮癣的小鼠模型中,HM/MN贴剂显著防止皮肤厚度降低的红斑,从而抑制牛皮癣的进展。进一步分析LN中的免疫细胞,在用HM/MN局部处理后,树突状细胞(DC)和T细胞的百分比降低。值得注意的是,通过检测甲氨蝶呤在LN中的积累增加,验证了使用纳米粒将甲氨蝶呤靶向递送至LN的可行性.此外,HM/MN贴片明显降低了皮肤中肿瘤坏死因子α和白细胞介素6的水平。
UNASSIGNED:结果表明,使用HM/MN贴剂治疗牛皮癣具有很高的疗效,并为透皮给药系统的机理提供了新的见解。
UNASSIGNED: Transdermal drug delivery provides a desirable alternative method of penetrating the skin for psoriasis treatment, by virtue of its ability to dampen the overactivation of immune cells and inflammation, while attenuating the detrimental effects of systemic administration. Lymph nodes (LNs), as a critical organ of the lymphatic and the acquired immune system, are suitable sites for drug homing to suppress the immune cells.
UNASSIGNED: In this context, we developed a microneedle (MN) patch that delivers nanodrugs locally to LNs for improving the antipsoriatic treatment. In this study, human serum albumin nanoparticles carrying methotrexate (HM) were synthesized and loaded into hyaluronic acid (HA)-based microneedles (HM/MN).
UNASSIGNED: The patch showed an excellent ability to pierce the skin, which enhanced drug delivery. In a mouse model of psoriasis, the HM/MN patch significantly prevented the erythema with decreased skin thickness, thus inhibiting the progression of psoriasis. Further analysis for immune cells in LNs, the percent of dendritic cells (DC) and T cells reduced after the local treatment with HM/MN. Notably, the feasibility of targeted delivery of methotrexate to LNs using nanoparticles was verified by detecting increased accumulation of methotrexate in LNs. In addition, the HM/MN patch pronouncedly decreased the levels of tumor necrosis factor α and interleukin 6 in the skin.
UNASSIGNED: The results suggested the high efficacy of using the HM/MN patch to treat psoriasis, and provided new insight into the mechanism of the transdermal drug delivery system.