■血管钙化是2型糖尿病患者心血管意外的主要原因。本研究旨在探讨碳水化合物对糖尿病ApoE-/-小鼠肠道菌群和主动脉钙化的影响。
■将糖尿病ApoE-/-小鼠随机分为4组:生酮饮食组,低碳水化合物饮食组,中等碳水化合物饮食组,和高碳水化合物饮食组。连续喂养小鼠6个月,血糖,每月监测血酮和体重。采用ELISA法检测脂质代谢指标和炎症因子。肠道屏障,动脉粥样硬化病变区域,血管钙化根据其形态进行分析。使用16SrRNA基因分析肠道微生物群。
■我们发现生酮饮食在改善血糖方面发挥了一些作用,脂质代谢,和炎症。生酮饮食可以在一定程度上改善肠道屏障,增加肠道细菌。与其他三组相比,Allobaculum属的相对丰度,生酮饮食组的Blautiaproducta和ClossumRamosum物种显着增加(P<0.05),对糖尿病ApoE-/-小鼠具有保护作用。
■生酮饮食可以延缓主动脉粥样硬化的发生,糖尿病ApoE-/-小鼠主动脉钙化和改善肠屏障功能。
UNASSIGNED: Vascular calcification is a major cause of cardiovascular accidents in patients with type 2 diabetes mellitus. This study aimed to investigate the impact of carbohydrates on gut microbiota and aortic calcification in diabetic ApoE-/- mice.
UNASSIGNED: The diabetic ApoE-/- mice were randomly divided into 4 groups: ketogenic diet group, low carbohydrate diet group, medium carbohydrate diet group, and high carbohydrate diet group. The mice were fed continuously for 6 months, with blood glucose, blood ketone and body weight monitored monthly. Lipid metabolism indicators and inflammatory factors were detected using ELISA. The intestinal barrier, atherosclerotic lesion areas, and vascular calcifications were analyzed based on their morphology. Gut microbiota was analyzed using 16S rRNA genes.
UNASSIGNED: We found that ketogenic diet played some roles improving glucose, lipid metabolism, and inflammation. Ketogenic diet could improve the intestinal barrier to some extent and increase intestinal bacteria. Compared to the other three groups, the relative abundance of genus Allobaculum, species Blautia producta and Clostridium Ramosum in the ketogenic diet group was significantly increased (P <0.05), which has protective effects in diabetic ApoE-/- mice.
UNASSIGNED: Ketogenic diet could delay the onset of aortic atherosclerosis, aortic calcification and improve intestinal barrier function in diabetic ApoE-/- mice.