真核基因组折叠成DNA环介导的结构维持染色体(SMC)复合物,如粘附蛋白,凝集素,Smc5/6这种组织在细胞周期中调节不同的DNA相关过程,比如转录,重组,隔离,DNA修复在G2阶段,SMC介导的DNA环与参与姐妹染色单体内聚(SCC)的粘附蛋白复合物共存。然而,SCC的建立和SMC介导的DNA环沿着染色质的形成之间的联系仍然未知。这里,我们表明SCC确实是粘附素介导的沿着G2/M酿酒酵母染色体的DNA环扩展的障碍。
Eukaryotic genomes are folded into DNA loops mediated by structural maintenance of chromosomes (SMC) complexes such as cohesin, condensin, and Smc5/6. This organization regulates different DNA-related processes along the cell cycle, such as transcription, recombination, segregation, and DNA repair. During the G2 stage, SMC-mediated DNA loops coexist with cohesin complexes involved in sister chromatid cohesion (SCC). However, the articulation between the establishment of SCC and the formation of SMC-mediated DNA loops along the chromatin remains unknown. Here, we show that SCC is indeed a barrier to cohesin-mediated DNA loop expansion along G2/M Saccharomyces cerevisiae chromosomes.