■真菌镰刀菌严重威胁热带土壤中的玉米作物。鉴于此,生物控制已成为降低杀菌剂成本和环境风险的有前途的策略。在这项研究中,我们的目的是测试来自三种Velezensis芽孢杆菌(CT02,IM14和LIS05)和一种Ottowii类芽孢杆菌(LIS04)的无细胞上清液(CFS)对轮虫的抗真菌活性,从而有助于制定有效的生物控制措施。
■这项研究采用了全面的方法。使用来自三种Velezensis芽孢杆菌(CT02,IM14和LIS05)和一种拟芽孢杆菌(LIS04)的无细胞上清液(CFS)测试了细菌菌株的抗真菌活性。UPLC-MS评估CFS以鉴定参与对黄萎病菌的抑制作用的主要生物活性分子。扫描电子显微镜(SEM)用于评估CFS对孢子和菌丝的影响,并进行基因组测序以鉴定参与生物防治的基因。这些稳健的方法确保了可靠性并验证了我们的发现。
■四种菌株的CFS表现出对真菌生长的显著抑制。UPLC-MS分析显示存在具有抗真菌活性的脂肽,包括由三个菌株的velezensis芽孢杆菌表达的表面活性素和fengycinsA和B,以及由菌株LIS05和IM14表达的iturinA。对于巴氏芽孢杆菌,镰刀素,ABCDE,检测到五种以前未报告的脂肽。扫描电子显微镜(SEM)表明,用CFS处理会导致轮虫菌丝的明显变形和破坏,除了在膜中形成空腔。基因组挖掘证实了编码UPLC-MS鉴定的脂肽的基因的存在,包括CTO2中的iturin基因.基因组测序显示CT02、IM14和LIS05分别属于不同菌株,LIS04属于Ottowii类芽孢杆菌,最近描述的一个物种。
■四种细菌菌株,包括三个被鉴定为velezensis芽孢杆菌的新菌株和一个最近被描述为penibacillusottowii的菌株,显示出作为控制真菌病的生物防治剂的巨大潜力。这一发现强调了我们研究的新颖性和潜在影响。
UNASSIGNED: The fungus Fusarium verticillioides significantly threatens maize crops in tropical soils. In light of this, biological control has emerged as a promising strategy to reduce fungicide costs and environmental risks. In this study, we aimed to test the antifungal activity of cell-free supernatant (CFS) from three Bacillus velezensis (CT02, IM14, and LIS05) and one Paenibacillus ottowii (LIS04) against F. verticillioides, thereby contributing to the development of effective biocontrol measures.
UNASSIGNED: The research employed a comprehensive approach. The antifungal activity of the bacterial strains was tested using cell-free supernatant (CFS) from three Bacillus velezensis (CT02, IM14, and LIS05) and one Paenibacillus ottowii (LIS04). The UPLC-MS evaluated the CFS to identify the main bioactive molecules involved in the inhibitory effect on F. verticillioides. Scanning electron microscopy (SEM) was used to assess the impact of CFS on spores and hyphae, and genome sequencing was conducted to identify the genes involved in biological control. These robust methodologies ensure the reliability and validate our findings.
UNASSIGNED: The CFS of the four strains demonstrated significant inhibition of fungal growth. The UPLC-MS analysis revealed the presence of
lipopeptides with antifungal activity, including surfactin and fengycins A and B expressed by the three strains of Bacillus velezensis and iturin A expressed by strains LIS05 and IM14. For Paenibacillus ottowii, fusaricidins, ABCDE, and five previously unreported
lipopeptides were detected. Scanning electron microscopy (SEM) showed that treatments with CFS led to significant distortion and breakage of the F. verticillioides hyphae, in addition to the formation of cavities in the membrane. Genome mining confirmed the presence of genes coding for the
lipopeptides identified by UPLC-MS, including the gene for iturin in CTO2. Genomic sequencing revealed that CT02, IM14, and LIS05 belong to different strains of Bacillus velezensis, and LIS04 belongs to Paenibacillus ottowii, a species recently described.
UNASSIGNED: The four bacterial strains, including three novel strains identified as Bacillus velezensis and one as the recently described species Paenibacillus ottowii, demonstrate significant potential as biocontrol agents for managing fungal disease. This finding underscores the novelty and potential impact of our research.