研究细胞色素c在含有50mol%1,2-二酰基磷脂酰甘油脂质[10:0、12:0、14:0、16:0、18:1(Δ9顺式)]的脂质体上和从脂质体上吸附和解吸的实验报告了pH7.4缓冲溶液中的1,2-二油酰基-sn-甘油-3-磷脂酰胆碱(DOPC)。荧光实验表明,细胞色素c对所研究的五种标记的50mol%PG脂质体系统具有相似的吸附亲和力。荧光恢复实验表明,加入>10×过量的未标记的100%1,2-二油酰基-sn-甘油-3-磷脂酰甘油(DOPG)脂质体后,细胞色素c解吸的程度取决于脂质的酰基链长度。解吸的程度也显示与温度无关,虽然范围很窄。细胞色素c从含有具有不同酰基链长度的PG脂质的脂质体中解吸的程度的差异归因于涉及延长的脂质锚定的结合基序响应于脂质包装应力的不同贡献。
Experiments investigating the adsorption and desorption of cytochrome c onto and from liposomes containing 50 mol% 1,2-diacylphosphatidylglycerol lipids [10:0, 12:0, 14:0, 16:0, 18:1(Δ9 cis)] with 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) in pH 7.4 buffered solutions of low to moderate ionic strength are reported. Fluorescence experiments show that cytochrome c has a similar adsorption affinity for the five labeled 50 mol% PG liposome systems investigated. Fluorescence recovery experiments reveal the extent of cytochrome c desorption upon the addition of >10× excess of unlabeled 100% 1,2-dioleoyl-sn-glycero-3-phosphatidylglycerol (DOPG) liposomes is dependent on the lipid\'s acyl chain length. The extent of desorption is also shown to be independent of temperature, albeit over a narrow range. The differences in the extent of cytochrome c desorption from liposomes containing PG lipids with different acyl chain lengths is attributed to the varying contribution of the binding motif involving the extended lipid anchorage in response to lipid packing stress.