KulicoidesoxystomaKieffer(双翅目:Ceratopogenidae)已经对几种虫媒病毒进行了媒介研究,原生动物和线虫,导致热带和亚热带的牲畜和野生反刍动物的死亡率和发病率。必须对与媒介物种相关的细菌群落进行了解。这项工作试图清点与这种重要的媒介物种相关的细菌群落。肠道微生物群的获得可能是父母的起源,而有些是在幼虫阶段通过喂食获得的。Culicoidesoxystoma具有产卵和幼虫生存的半水生生命周期策略。与产氧菌相关的细菌在整个(I)生命阶段进行了比较:卵,幼虫幼虫,蛹,成年:从实验室殖民地获得的雄性和雌性;(ii)实地收集的成年:雄性和年龄分级的雌性;(iii)天然育种基质和人工饲养基质。通过16SrRNA的Sanger测序鉴定培养依赖性细菌,在血琼脂上筛选溶血性细菌。结果表明,Firmicutes和Proteobacteria是主要的Phyla,其中芽孢杆菌属。是生命阶段中最丰富的。在整个生活史上,蜡样芽孢杆菌,短小芽孢杆菌,热带芽孢杆菌,溶血芽孢杆菌。和类芽孢杆菌。被常规检索。在实验室饲养的标本中检测到蜡状芽孢杆菌和粪产碱杆菌,并在自然育种地点和饲养培养基之间共享。从被困在两个地点的成年人那里,B.蜡质,弯曲芽孢杆菌,A.粪肠,屎肠球菌和假单胞菌。被隔离。与该载体相关的细菌物种可能会影响各种生理特性,例如矢量容量,消化和幼虫发育,需要进一步调查。
Culicoides oxystoma Kieffer (Diptera: Ceratopogonidae) has been vectoring several arboviruses, protozoa and nematodes, leading to mortality and morbidity of livestock and wild ruminants in the tropics and subtropics. Insight into the bacterial communities associated with the vector species must be worked out. This work tries to inventorize the bacterial communities associated with this important vector species. Acquisition of gut microbiota may be the parental origin, while some are obtained through feeding during larval stages. Culicoides oxystoma possesses semi-aquatic life cycle strategies for egg-laying and larval survival. The bacteria associated with C. oxystoma were compared throughout (i) life stages: egg, larval instars, pupa, adult: male and female obtained from laboratory colony; (ii) field-collected adult: male and age-graded females; and (iii) natural breeding substrate and artificial rearing substrate. The culture-dependent bacteria were identified by Sanger sequencing of 16S rRNA, and haemolytic bacteria were screened on blood agar. Results show that Firmicutes and Proteobacteria are the predominant Phyla, of which Bacillus spp. was the most abundant across the life stages. Across the life history, Bacillus cereus, Bacillus pumilus, Bacillus tropicus, Lysinibacillus sp. and Paenibacillus sp. were retrieved routinely. Bacillus cereus and Alcaligenes faecalis were detected in the lab-reared specimens and shared between the natural breeding site and rearing medium. From the adults trapped across two locations, B. cereus, Bacillus flexus, A. faecalis, Enterococcus faecium and Pseudomonas sp. were isolated. The bacterial species associated with this vector may influence various physiological traits, such as vectorial capacity, digestion and larval development, which need further investigation.