背景:亚马逊海龟的血液学,例如Arrau乌龟(Podocnemisexpansa),在文献中没有得到很好的描述。参考如何收集和分析血液样本是这些外来动物的医学实践的基础。
目的:本研究的目的是评估亚马逊Arrau海龟的血液学参数,以确定最佳的抗凝剂(EDTA,柠檬酸盐或肝素钠)和细胞学染色(Giemsa,快速全景,或Rosenfeld)用于此物种。
方法:收集8只海龟的血样。用没有抗凝剂的血液做了三次血涂片,其余的样品分布在三个试管中,含有EDTA,柠檬酸盐还有肝素钠,用于红细胞图和白细胞图分析。
结果:用EDTA抗凝的所有血液样品显示明显溶血。用柠檬酸盐收集的血液显示最小的溶血,用肝素钠收集的血液没有任何溶血。由于强烈的溶血,一些分析未对含有EDTA的血液进行。获得了红细胞变量,并在肝素中的血液样品中显示出更好的结果。没有抗凝剂的血涂片适用于三种染色类型的区分白细胞计数,而任何抗凝剂的血液都难以区分白细胞。
结论:肝素钠显示出最好的结果,应作为Pexpansa血液学研究的首选抗凝剂。所有细胞学染色都是有效的,允许充分识别不同的细胞组。白细胞分化得到了保证,并且使用没有抗凝剂的血液涂片具有更好的对比度。
BACKGROUND: The hematology of turtles of the Amazon, such as the Arrau turtle (Podocnemis expansa), has not been well described in the literature. Referencing how to collect and analyze blood samples is fundamental for the medical practice of these exotic animals.
OBJECTIVE: The aim of this study was to evaluate the hematologic parameters of Arrau turtles of the Amazon to determine the best anticoagulant (EDTA, citrate, or sodium heparin) and cytologic stain (Giemsa, Quick Panoptic, or Rosenfeld) for use with this species.
METHODS: Blood samples from eight turtles were collected. Three blood smears were made using blood without anticoagulant, and the rest of the sample was distributed in three tubes, containing EDTA, citrate, and sodium heparin, for erythrogram and
leukogram analyses.
RESULTS: All blood samples anticoagulated with EDTA showed marked hemolysis. Blood collected with citrate showed minimal hemolysis, and blood collected with sodium heparin did not have any hemolysis. Some analyses were not performed on blood containing EDTA because of the intense hemolysis. The erythrocyte variables were obtained and showed better results for blood samples in heparin. Blood smears without anticoagulant were appropriate for differential leukocyte counts with the three stain types, while blood with any of the anticoagulants made it difficult to differentiate leukocytes.
CONCLUSIONS: Sodium heparin showed the best results and should be the anticoagulant of choice for hematologic studies in P expansa. All cytologic stains were efficient, allowing adequate identification of distinct cell groups. Leukocyte differentiation was assured and had better contrast using blood smears made from blood without an anticoagulant.