UNASSIGNED: To explore the best centrifuge condition for preparing rabbit leukocyte-poor platelet-rich plasma (LP-PRP) by using single centrifugation method.
UNASSIGNED: Sixteen healthy New Zealand rabbits, aged 3-4 months, were utilized in the investigation. A total of 15 mL anticoagulated blood was extracted from the central ear artery of each rabbit, with a repeat of the blood collection procedure after 1 and 2 months. The obtained blood specimens were individually subjected to centrifugation at a radius of 16.7 cm and speeds of 1 200, 1 300, 1 400, and 1 500 r/min (equivalent to centrifugal forces of 269× g, 315× g, 365× g, and 420× g) for durations of 2, 3, 4, and 5 minutes, resulting in a total of 16 groups. Following centrifugation, collect plasma from each group to a distance of 1.5 mL from the separation plane. The volumes, platelet enrichment coefficient, and platelet recovery rates of LP-PRP in each group, under varying centrifugation conditions, were methodically computed and subsequently compared.
UNASSIGNED: The volume of LP-PRP obtained under all centrifugation conditions ranged from 1.8 to 7.6 mL. At a consistent centrifugal speed, an extension of centrifugation time leaded to a significant increase in the volume of LP-PRP, accompanied by a declining trend in the platelet enrichment coefficient of LP-PRP. When centrifuged for 2 minutes, the volume of LP-PRP at speeds of 1 200 and 1 300 r/min was less than 2.0 mL, while the volume of LP-PRP obtained under other conditions was more than 2.0 mL. When centrifuged for 4 and 5 minutes, the volume of LP-PRP obtained at each speed was more than 4 mL. LP-PRP with a platelet enrichment coefficient more than 2.0 could be prepared by centrifuging at 1 200 r/min for each time group and 1 300 r/min for 2 and 3 minutes, and the highest LP-PRP platelet enrichment coefficient could be obtained by centrifugation for 2 minutes at a speed of 1 200 r/min. The platelet recovery rates of LP-PRP obtained by centrifugation at 1 200 r/min for 4 and 5 minutes, as well as centrifugation at 1 400 r/min for 5 minutes, were both greater than 60%. There was no significant difference between the groups when centrifuged at 1 200 r/min for 4 and 5 minutes ( P>0.05).
UNASSIGNED: In the process of preparing rabbit LP-PRP using a single centrifugation method, collecting 15 mL of blood and centrifuging at a radius of 16.7 cm and speed of 1 200 r/min for 4 minutes can prepare LP-PRP with a volume exceeding 2.0 mL, platelet enrichment coefficient exceeding 2.0, and platelet recovery rate exceeding 60%. This centrifugal condition can achieve the optimal LP-PRP action parameters in the shortest possible time.
UNASSIGNED: 探究使用一次离心法制备兔贫白细胞富血小板血浆(leukocyte-poor platelet-rich plasma,LP-PRP)的最佳离心条件。.
UNASSIGNED: 取16只3~4月龄健康新西兰兔,于兔耳背中央动脉采集15 mL抗凝血,分别于1、2个月后重复取血步骤。将所取血液标本于离心半径16.7 cm分别以转速1 200、1 300、1 400、1 500 r/min(对应离心力分别为269× g、315× g、365× g、420× g)下离心2、3、4、5 min,共计16组。各组离心后采集分离平面1.5 mL以上的血浆,计算并比较不同离心条件下各组LP-PRP的体积、血小板富集系数及血小板回收率。.
UNASSIGNED: 所有离心条件下获得的LP-PRP体积范围为1.8~7.6 mL。相同离心转速时,随离心时间延长,LP-PRP体积显著增大,同时LP-PRP血小板富集系数呈降低趋势。离心2 min时,1 200、1 300 r/min转速下LP-PRP体积<2.0 mL,其余条件下获得的LP-PRP体积均≥2.0 mL;离心4、5 min时,各转速下获得的LP-PRP体积均≥4.0 mL。1 200 r/min转速下各离心时间组和1 300 r/min转速下离心2、3 min组LP-PRP血小板富集系数均>2.0,其中1 200 r/min转速下离心2 min能够获得最高的LP-PRP血小板富集系数。1 200 r/min转速下离心4、5 min与1 400 r/min转速下离心5 min获得的LP-PRP血小板回收率均>60%,1 200 r/min转速下离心4、5 min组间比较差异无统计学意义( P>0.05)。.
UNASSIGNED: 采用一次离心法制备兔LP-PRP时,采集15 mL血液以离心半径16.7 cm、1 200 r/min转速下离心4 min即可制备体积>2.0 mL、血小板富集系数>2.0且血小板回收率>60%的LP-PRP,在最短时间达到LP-PRP的最佳作用参数。.