角质层(SC)对药物的局部给药具有一定的局限性。这可以使用渗透促进剂(PE)如萜烯(TP)来克服。SC对于维持皮肤屏障也是至关重要的,并且由两个层状结构组成:短周期性相(SPP)和长周期性相(LPP)。在这项研究中,我们监测了人类SC的X射线衍射峰的变化,TP应用后30min(neroridol,1,8-cineol,和d-柠檬烯)。随着Nerolidol的应用,对于SPP的层状结构,在小角度衍射峰位置没有观察到显著的变化,但综合强度下降。相反,当使用1,8-桉树脑和d-柠檬烯时,与1,8-桉树脑相比,观察到d-柠檬烯的SPP衍射峰的峰宽变宽的较低角度峰位移,d-柠檬烯的峰移和宽度展宽程度大于1,8-桉树脑。当应用1,8-桉树脑和d-柠檬烯时,LPP的衍射峰消失。这些结果表明,SC和TP之间的相互作用程度因分子种类而异,d-柠檬烯和1,8-桉树脑通过破坏SPP和LPP的层状结构表现出渗透增强作用,申请后立即。
The stratum corneum (SC) presents certain limitations for topical administration of medication, which can be overcome using penetration enhancers (PEs) such as terpene (TP). The SC is also crucial for maintaining the skin barrier and consists of two lamellar structures: the short periodicity phase (SPP) and long periodicity phase (LPP). In this study, we monitored changes in the X-ray diffraction peaks of the human SC, 30 min after TP application (neroridol, 1,8-cineol, and d-limonene). With the application of nerolidol, no significant changes were observed in the small-angle diffraction peak positions for the lamellar structure of SPP, but the integrated intensity decreased. On the contrary, when applying 1,8-cineole and d-limonene, a lower angle peak shift with broadening of the peak width of SPP diffraction peaks was observed for d-limonene than for 1,8-cineole, and the degree of peak shift and width broadening was greater for d-limonene than for 1,8-cineole. The diffraction peaks of LPP disappeared when 1,8-cineole and d-limonene were applied. These results indicate that the degree of interaction between the SC and TP differs depending on the molecular species, and d-limonene and 1,8-cineole exhibit penetration-enhancing via lamellar structure disruption of both SPP and LPP, immediately after application.