四个杆状,不活动,非孢子形成,兼性厌氧,革兰氏染色阳性乳酸菌,指定为EB0058T,SCR0080,LD0937T和SCR0063T,从不同的玉米和草青贮样品中分离。使用多相方法对分离的菌株进行了表征,并通过16SrRNA基因序列分析将EB0058T和SCR0080鉴定为玉米乳杆菌。基于全基因组序列的表征,EB0058T和SCR0080被分离为来自玉米乳杆菌DSM20178T的独特进化枝,与CECT9104和UD2202一起,其基因组序列可从NCBIGenBank获得。新亚组中的平均核苷酸同一性(ANI)值为99.9%,数字DNA-DNA杂交(dDDH)值为99.3-99.9%,分别。相比之下,将新的亚组与公开可用的玉米乳杆菌菌株的基因组序列进行比较,包括DSM20178T型应变,显示dDDH值为70.2-72.5%,ANI值为96.2-96.6%。根据它们的化学分类学,表型和系统发育特征,EB0058T和SCR0080代表玉米乳杆菌的新亚种。名称乳杆菌玉米亚种。silagei亚科。11月。提出的应变为EB0058T(=DSM116376T=NCIMB15474T)。根据16SrRNA基因测序结果,LD0937T和SCR0063T是乳杆菌组的成员。分离物LD0937T和SCR0063T之间的dDDH值为67.6%,低于70%的物种门槛,清楚地表明这两个分离株属于不同的物种。对于这两种菌株,全基因组测序显示,乳杆菌组的近亲分别为胡氏乳杆菌DSM115425(dDDH66.5和65.9%)和干酪乳杆菌DSM20011T(dDDH64.1和64.9%).基于基因组,在这项研究中获得的化学分类和形态学数据,两个新颖的物种,副乳杆菌。11月。和苯乙烯乳杆菌。11月。提出了菌株类型为LD0937T(=DSM116105T=NCIMB15471T)和SCR0063T(=DSM116297T=NCIMB15473T),分别。
Four rod-shaped, non-motile, non-spore-forming, facultative anaerobic, Gram-stain-positive lactic acid bacteria, designated as EB0058T, SCR0080, LD0937T and SCR0063T, were isolated from different corn and grass silage samples. The isolated strains were characterized using a polyphasic approach and EB0058T and SCR0080 were identified as Lacticaseibacillus zeae by 16S rRNA gene sequence analysis. Based on whole-genome sequence-based characterization, EB0058T and SCR0080 were separated into a distinct clade from Lacticaseibacillus zeae DSM 20178T, together with CECT9104 and UD2202, whose genomic sequences are available from NCBI GenBank. The average nucleotide identity (ANI) values within the new subgroup are 99.9 % and the digital DNA-DNA hybridization (dDDH) values are 99.3-99.9 %, respectively. In contrast, comparison of the new subgroup with publicly available genomic sequences of L. zeae strains, including the type strain DSM 20178T, revealed dDDH values of 70.2-72.5 % and ANI values of 96.2-96.6 %. Based on their chemotaxonomic, phenotypic and phylogenetic characteristics, EB0058T and SCR0080 represent a new subspecies of L. zeae. The name Lacticaseibacillus zeae subsp. silagei subsp. nov. is proposed with the type strain EB0058T (=DSM 116376T=NCIMB 15474T). According to the results of 16S rRNA gene sequencing, LD0937T and SCR0063T are members of the Lacticaseibacillus group. The dDDH value between the isolates LD0937T and SCR0063T was 67.6 %, which is below the species threshold of 70 %, clearly showing that these two isolates belong to different species. For both strains, whole genome-sequencing revealed that the closest relatives within the Lacticaseibacillus group were Lacticaseibacillus huelsenbergensis DSM 115425 (dDDH 66.5 and 65.9 %) and Lacticaseibacillus casei DSM 20011T (dDDH 64.1 and 64.9 %). Based on the genomic, chemotaxonomic and morphological data obtained in this study, two novel species, Lacticaseibacillus parahuelsenbergensis sp. nov. and Lacticaseibacillus styriensis sp. nov. are proposed and the type strains are LD0937T (=DSM 116105T=NCIMB 15471T) and SCR0063T (=DSM 116297T=NCIMB 15473T), respectively.