一品红根的化学研究。导致分离出五种未描述的拉布丹二萜化合物“(4S,5S,9R,10S,13R)-18-O-没食子酰-labda-8(17),14(15)-二烯-13-醇;(4S,5S,9R,10S,13R)-13-羟基-labda-8(17),14(15)-dien-18-one;(4S,5S,9R,10S,13R)-18-O-乙酰基-labda-8(17),14(15)-二烯-13-醇;(4S,5S,9R,10S)-labda-8(17),13(16)、14(15)-三烯-18-醇;(5R,6R,9R,10S,13R)-labda-8(17),14(15)-二烯-6,13-二醇\“,两种未描述的匹马兰二萜化合物(2R,5S,9R,10S,12R,13R)-2,12-二羟基-异比马拉-7,15-二烯-3-酮;(5S,9R,10S,12R,13R)-2,12-二羟基-异西马拉-1,7,15-三烯-3-酮)\“,连同九种已知的二萜,包括三种匹马环型\“(3β,11α,13α)-3,11-二羟基西番莲-7,15-二烯-2,12-二酮;(11R,12S)-2,11,12-三羟基-ent-isopimara-1,7,15-三烯-3-酮;isopimara-7,15-dien-3β-ol)“,五松香烷型\"日光素内酯A-C;日光素内酯E;日光素内酯I",和一个lathyrane类型的“jolkinolB”。通过HRESIMS分析阐明了这些化合物的结构,1DNMR,2DNMR,和X射线衍射。在体外评估了这16种化合物对三种人癌细胞系的细胞毒性活性,U-937,LOVO,K-562JolkinolB对U-937和LOVO细胞系的IC50为3.60μM和8.44μM,(4S,5S,9R,10S,13R)-18-O-没食子酰-labda-8(17),14(15)-dien-13-ol对U-937细胞系显示的IC50为5.92μM,isopimara-7,15-dien-3β-ol对K-562细胞系的IC50为0.87μM。
Chemical investigation of the roots of Euphorbia pekinensis Rupr. led to the isolation of five undescribed labdane diterpenoids \"(4S, 5S, 9R, 10S, 13R)-18-O-galloyl-labda-8(17), 14(15)-dien-13-ol; (4S, 5S, 9R, 10S, 13R)-13-hydroxy-labda-8(17), 14(15)-dien-18-one; (4S, 5S, 9R, 10S, 13R)-18-O-acetyl-labda-8(17), 14(15)-dien-13-ol; (4S, 5S, 9R, 10S)-labda-8(17), 13(16), 14(15)-trien-18-ol; (5R, 6R, 9R, 10S, 13R)-labda-8(17), 14(15)-dien-6,13-diol\", two undescribed pimarane diterpenoids \"(2R, 5S, 9R, 10S, 12R, 13R)-2,12-dihydroxy-isopimara-7,15-dien-3-one; (5S, 9R, 10S, 12R, 13R)-2, 12-dihydroxy-isopimara-1, 7, 15-trien-3-one)\", together with nine known diterpenoids, including three pimarane-type \"(3β,11α,13α)-3,11-dihydroxypimara-7,15-diene-2,12-dione; (11R, 12S)-2,11,12-trihydroxy-ent-isopimara-1,7,15-trien-3-one; isopimara-7,15-dien-3β-ol)\", five abietane-type \"helioscopinolide A-C; helioscopinolide E; helioscopinolide I″, and one lathyrane-type \"jolkinol B\". The structures of these compounds were elucidated by analysis of HRESIMS, 1D NMR, 2D NMR, and X-ray diffraction. These sixteen compounds were evaluated for cytotoxic activity in vitro against three human cancer cell lines, U-937, LOVO, and K-562. Jolkinol B exhibited IC50 of 3.60 μM and 8.44 μM against U-937 and LOVO cell lines, (4S, 5S, 9R, 10S, 13R)-18-O-galloyl-labda-8(17), 14(15)-dien-13-ol displayed IC50 of 5.92 μM against U-937 cell lines, isopimara-7,15-dien-3β-ol showed IC50 of 0.87 μM against K-562 cell lines.