Introduction.十羧基球藻是肠杆菌的成员,通常被认为是机会病原体。最近的报道强调了十二羧基乳杆菌是一种具有毒力和抗性决定因子的新兴病原体。差距声明。关于从环境中分离出的十八羧基乳杆菌菌株的毒力和抗性决定因素的信息很少,食物,和临床样本。瞄准.为了确定从环境中分离的十二羧基乳杆菌菌株中抗性和毒力决定子和质粒特征的存在,食物,和临床样本,以及它们的系统发育关系。结果。所有测试的菌株均对β-内酰胺类和喹诺酮类具有抗性,但对氨基糖苷类和硝基呋喃类敏感。然而,尽管磷霉素抗性被认为是十四羧基乳杆菌的特征性状,抗性表型仅在50%的菌株中观察到;BLATEM是最普遍的BLEE基因(70%),而在60%的菌株中观察到喹诺酮qnrB基因。在菌株中差异观察到毒力基因,与粘附相关的基因是最丰富的,其次是毒素基因。最后,所有菌株都携带一到七个从7到125kbps的质粒带,并具有几个质粒成瘾系统,如ParDE,VagCD,和CcdAB在80%的菌株中。Conclusions.十二羧基乳杆菌是一种重要的新兴病原体,可能具有抗性和毒力基因。此外,它具有可移动的遗传元件,可能有助于遗传决定子传播到其他细菌属。
Introduction. Leclercia adecarboxylata is a member of Enterobacterales, often considered an opportunistic pathogen. Recent reports have highlighted L. adecarboxylata as an emerging pathogen harbouring virulence and resistance determinants.Gap statement. Little information exists on virulence and resistance determinants in L. adecarboxylata strains isolated from environmental, food, and clinical samples.Aim. To determine the presence of resistance and virulence determinants and plasmid features in L. adecarboxylata strains isolated from environmental, food, and clinical samples, as well as their phylogenetic relationship.Results. All strains tested showed resistance to β-lactams and quinolones but were sensitive to aminoglycosides and nitrofurans. However, even though fosfomycin resistance is considered a characteristic trait of L. adecarboxylata, the resistance phenotype was only observed in 50 % of the strains; bla TEM was the most prevalent BLEE gene (70 %), while the quinolone qnrB gene was observed in 60 % of the strains. Virulence genes were differentially observed in the strains, with adhesion-related genes being the most abundant, followed by toxin genes. Finally, all strains carried one to seven plasmid bands ranging from 7 to 125 kbps and harboured several plasmid addiction systems, such as ParDE, VagCD, and CcdAB in 80 % of the strains.Conclusions. L. adecarboxylata is an important emerging pathogen that may harbour resistance and virulence genes. Additionally, it has mobilizable genetic elements that may contribute to the dissemination of genetic determinants to other bacterial genera.