背景:Kratom(来自MitragynaspeciosaKorth的叶子。;茜草科)是一种以其镇痛特性和精神作用而闻名的草药。泰国的Kratom目前是合法的;然而,它在一些国家被禁止,被认为是麻醉植物。
目标:我们的目标是建立可靠的,简单,以及通过高效薄层色谱法(HPTLC)和密度测定法来定量Kratom叶及相关产品中的mitragynine的快速方法。
方法:开发了一种光密度HPTLC方法,并在特异性方面进行了验证,线性度检测限(LOD),定量限(LOQ),准确度,精度,和鲁棒性。kratom叶子的指纹,Mitragynaspp.,和相关产品的建设。
结果:对于HPTLC,将样品应用于硅胶60F254板,流动相包括正己烷,乙酸乙酯,和三乙胺(1:1:0.15,v/v/v)。在226nm波长的紫外光下进行光密度检测。经过验证的方法范围为14.31-143.10μg/mL,相关系数为0.9993。加标回收率在98.3%-100.9%范围内,LOD和LOQ分别为3.80和11.53μg/mL,分别。用开发的方法分析Kratom样品,与高效液相色谱-二极管阵列检测(HPLC-DAD)方法相比,相关系数为0.9641。HPTLC指纹显示出独特的图案,促进不同植物部位和Mitragynaspp之间的区分。
结论:所建立的方法具有简单的优点,易用性,和分析的速度,作为kratom叶及其相关产品中mitragynine定量的实用替代品。
BACKGROUND: Kratom (leaves from Mitragyna speciosa Korth.; Rubiaceae) is a herbal medicine known for its analgesic properties and psychoactive effects.
Kratom in Thailand is currently legal; however, it is prohibited in some countries and considered a narcotic plant.
OBJECTIVE: Our aim was to establish a reliable, simple, and rapid method for quantifying mitragynine in
Kratom leaves and related products through a combination of high-performance thin-layer chromatography (HPTLC) and densitometry.
METHODS: A densitometric HPTLC method was developed and validated in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, and robustness. The fingerprints of
kratom leaves, Mitragyna spp., and related products were constructed.
RESULTS: For HPTLC, samples were applied to silica gel 60 F254 plates, and the mobile phase comprised n-hexane, ethyl acetate, and triethylamine (1:1:0.15, v/v/v). Densitometric detection was carried out under ultraviolet light at a wavelength of 226 nm. The validated method exhibited a range of 14.31-143.10 μg/mL, yielding a correlation coefficient of 0.9993. Spiked recovery rates were within a range of 98.3%-100.9%, and the LOD and LOQ were 3.80 and 11.53 μg/mL, respectively. Kratom samples were analyzed with the developed method, and the correlation coefficient was 0.9641, compared to the high-performance liquid chromatography-diode-array detection (HPLC-DAD) method. The HPTLC fingerprints displayed a distinctive pattern, facilitating discrimination among different plant parts and Mitragyna spp.
CONCLUSIONS: The established method offers the advantages of simplicity, ease of use, and speed of analysis, serving as a practical alternative for mitragynine quantification in
kratom leaf and its related products.