化脓性链球菌或A组链球菌(GAS)仍然是世界上一个重要的传染性问题,特别是在中低收入地区。此外,最近在高收入地区发现了侵袭性GAS感染(iGAS)的激增.然而,到目前为止,没有疫苗可用。寻找良好的疫苗抗原和了解毒力因子在GAS感染中的作用受到了阻碍,在某种程度上,通过技术困难来转化许多不同的菌株并产生敲除突变体。使用colE1型质粒作为自杀载体,我们已经建立了一种方法,允许在3天内产生GAS的非极性突变体。
目的:A群链球菌(GAS)是人类的主要病原菌,引起的疾病范围从皮肤和咽部上皮的轻度和浅表感染到严重的全身性和侵入性疾病。自2022年6月以来,几个欧洲国家,美国,澳大利亚正面临着危及生命的侵袭性GAS感染的热潮。寻找良好的疫苗抗原和了解毒力因子在GAS感染中的作用受到了阻碍,在某种程度上,通过技术困难来转化许多不同的GAS菌株并产生敲除突变体。此外,这些工具必须适应各种不同的菌株,因为GAS分为260多种EMM型(M型)。我们已经建立了一种方法,允许在3天内和不同背景下产生GAS的非极性突变体,这与以前发布的协议形成了鲜明对比。
Streptococcus pyogenes or Group A Streptococcus (GAS) remains a significant infectious problem around the world, particularly in low- and middle-income settings. Moreover, a recent invasive GAS infection (iGAS) upsurge has been observed in high-income settings. However, to date, no vaccine is available. Finding a good vaccine antigen and understanding the role of virulence factors in GAS infections have been hampered, in part, by technical difficulties to transform the many different strains and generate
knockout mutants. Using colE1-type plasmid as a suicide vector, we have set up a method allowing the generation of non-polar mutants of GAS in 3 days.
OBJECTIVE: Group A Streptococcus (GAS) is a major human pathogen, causing diseases ranging from mild and superficial infections of the skin and pharyngeal epithelium to severe systemic and invasive diseases. Since June 2022, several European countries, the US, and Australia are facing an upsurge of invasive life-threatening GAS infections. Finding a good vaccine antigen and understanding the role of virulence factors in GAS infections have been hampered, in part, by technical difficulties to transform the many different GAS strains and generate
knockout mutants. Moreover, these tools must be adapted to a large range of different strains, since GAS are divided into more than 260 emm-types (M-type). We have set up a method allowing the generation of non-polar mutants of GAS in 3 days and in diverse backgrounds, which contrasts with previously published protocols.