目的:Klotho是一种与人类衰老密切相关的蛋白质。可溶性Klotho(S-Klotho)是一种循环蛋白,其水平随着全身性炎症而降低。血小板/高密度脂蛋白胆固醇比值(PHR)新出现的炎症指数,S-Klotho浓度尚不清楚。此外,已证实平均血小板体积与S-Klotho浓度呈显著负相关,但血小板计数(PC)与S-Klotho浓度之间的关系尚未报道。
方法:检索了2007年至2016年五个周期内参加国家健康与营养调查(NHANES)的个人的数据进行分析。线性回归,两分段线性回归,和有限三次样条(RCS)方法用于分析PHR指数及其成分与S-Klotho浓度的关联。此外,进行亚组分析和效应修正试验。
结果:共有11,123名参与者(5463名男性(48.17%)),平均年龄为56.2岁,包括在内。完全调整后,PHR最高四分位数组(β:-51.19,95%CI:-75.41至-26.97,P<0.001)和PC最高四分位数组(β:-72.34,95%CI:-93.32至-51.37,P<0.0001)的参与者的S-Klotho水平显着低于各自的最低四分位数组,四组间呈明显下降趋势(P分别为趋势<0.05)。然而,高密度脂蛋白胆固醇(HDL-C)浓度与S-Klotho浓度无显著相关性.RCS显示PHR和PC与S-Klotho浓度呈非线性相关;两分段线性回归显示拐点分别为175.269和152,这些关联在拐点之后略有减弱。根据亚组分析,肝病状态增强了PC和S-Klotho浓度之间的关联。
结论:PHR和PC均与S-Klotho浓度呈显著负相关,这些关联是非线性的。HDL-C和S-Klotho浓度之间没有显著关联。肝病状态增强PC和S-Klotho浓度之间的负相关性,具体机制值得进一步探索。
OBJECTIVE: Klotho is a protein that is closely related to human aging. Soluble
Klotho (S-
Klotho) is a circulating protein, and its level decreases in response to systemic inflammation. The relationship between the platelet/high-density lipoprotein cholesterol ratio (PHR), an emerging inflammatory index, and S-
Klotho concentrations is still unclear. In addition, the mean platelet volume has been confirmed to have a significant negative association with S-Klotho concentrations, but the relationship between the platelet count (PC) and S-
Klotho concentrations has not yet been reported.
METHODS: Data from individuals who participated in the National Health and Nutrition Examination Survey (NHANES) during the five cycles from 2007 to 2016 were retrieved for analysis. Linear regression, two-piecewise linear regression, and restricted cubic spline (RCS) methods were used to analyze the associations of the PHR index and its components with S-Klotho concentrations. In addition, subgroup analysis and effect modification tests were conducted.
RESULTS: A total of 11,123 participants (5463 men (48.17%)), with an average age of 56.2 years, were included. After full adjustment, the S-Klotho levels of participants in the highest quartile group of PHR (β: -51.19, 95% CI: -75.41 to -26.97, P < 0.001) and the highest quartile group of PC (β: -72.34, 95% CI: -93.32 to -51.37, P < 0.0001) were significantly lower than those in their respective lowest quartile groups, and a significant downward trend was presented among the four groups (P for trend < 0.05, respectively). However, high-density lipoprotein cholesterol (HDL-C) concentrations were not significantly associated with S-Klotho concentrations. RCS revealed that the PHR and PC were nonlinearly associated with S-Klotho concentrations; two-piecewise linear regression revealed that the inflection points were 175.269 and 152, respectively, and that these associations slightly weakened after the inflection point. According to the subgroup analysis, liver disease status enhanced the association between the PC and S-Klotho concentrations.
CONCLUSIONS: Both the PHR and PC were significantly negatively associated with S-Klotho concentrations, and these associations were nonlinear. There was no significant association between HDL-C and S-
Klotho concentrations. Liver disease status enhances the negative association between the PC and S-Klotho concentrations, and the specific mechanism deserves further exploration.