■皮肤鳞状细胞癌(cSCC),一种常见的皮肤恶性肿瘤,经常发生在暴露的地方,手术切除后患者的外观会受到影响。本研究试图通过生物信息学分析筛选cSCC的关键基因,并探讨这些基因在cSCC中的临床意义和可能的潜在机制。
■我们从基因表达综合数据库中筛选了cSCC和正常皮肤组织之间的差异表达基因(DEG),进行功能富集和蛋白质相互作用网络分析,并使用Cytoscape软件识别关键基因。免疫组化证明了该基因的表达。
■共筛选了164个DEG,功能富集分析表明,DEGs在脱氧核糖核酸复制和细胞周期途径中显著富集。通过构建蛋白质-蛋白质相互作用网络,kinesin家族成员11(KIF11),极光激酶A(AURKA),微型染色体维持复合物组件2(MCM2),微型染色体维持10复制起始因子(MCM10),无牙E3泛素蛋白连接酶同源物(DTL)被鉴定为5个具有最高连通性的关键基因。KIF11的表达,AURKA,和MCM2通过免疫组织化学进行了研究。与正常皮肤组织相比,cSCC组织中KIF11和MCM2蛋白的阳性率分别为70.0%和90.0%,分别,差异有统计学意义(P<0.05)。cSCC和正常皮肤组织中AURKA蛋白表达的阳性率分别为13.9%和0%,分别,但差异无统计学意义。上述3个关键基因之间无相关性。
■KIF11和MCM2在cSCC中高表达,可能与肿瘤发生有关,并代表了cSCC临床诊断和治疗的新靶点。
UNASSIGNED: Cutaneous squamous cell carcinoma (cSCC), a common skin malignancy, often occurs at exposed sites, and patients\' appearance after surgical resection can be affected. This study sought to screen the key genes of cSCC via a bioinformatics analysis and explore the clinical significance and possible potential mechanisms of these genes in cSCC.
UNASSIGNED: We screened differentially expressed genes (DEGs) between cSCC and normal skin tissues from the Gene Expression Omnibus database, performed functional enrichment and protein interaction network analyses, and used Cytoscape software to identify the key genes. The expression of the genes was proved by immunohistochemistry.
UNASSIGNED: A total of 164 DEGs were screened, and the functional enrichment analysis showed that the DEGs were significantly enriched in deoxyribonucleic acid replication and the cell-cycle pathway. By constructing a protein-to-protein interaction network, kinesin family member 11 (KIF11), aurora kinase A (AURKA), minichromosome maintenance complex component 2 (MCM2), minichromosome maintenance 10 replication initiation factor (MCM10), and denticleless E3 ubiquitin protein ligase homolog (DTL) were identified as 5 key genes with the highest connectivity. The expression of KIF11, AURKA, and MCM2 were investigated by immunohistochemistry. Compared to the normal skin tissues, the positive rates of the KIF11 and MCM2 proteins in the cSCC tissues were 70.0% and 90.0%, respectively, and the difference was statistically significant (P<0.05). The positive rates of AURKA protein expression in the cSCC and normal skin tissues were 13.9% and 0%, respectively, but the difference was not statistically significant. There was no correlation between the above-mentioned 3 key genes.
UNASSIGNED: KIF11 and MCM2 were highly expressed in cSCC, and may be involved in tumorigenesis, and represent novel targets for the clinical diagnosis and treatment of cSCC.