背景:茄子。(茄科)是巴西的药用植物,传统上被称为“panaceia”。它的民间名称可能是由于其在传统医学中的广泛应用,包括治疗溃疡。
目的:使用体内胃溃疡模型评估S.cernuum的乙醇提取物(ESC)及其主要分离化合物的胃保护活性。
方法:通过在乙醇:水(7:3)中浸渍随后渗滤S.cernuum的干燥和粉末化的叶获得ESC提取物。通过应用各种制备色谱技术分离提取物中的主要化合物。使用不同的胃溃疡诱导模型在小鼠中评估胃保护活性。使用琼脂孔扩散和肉汤微量稀释方法进行抗幽门螺杆菌活性。
结果:ESC提取物在HCl/EtOH诱导的急性胃溃疡试验中显示出胃保护作用,非甾体抗炎药,和乙酸诱导的慢性溃疡方案。结果还表明,ESC提取物诱导的胃保护作用与一氧化氮和内源性巯基的活性有关,是重要的胃保护因子.在所测试的浓度下,ESC提取物和生物碱Cernumidine不显示抗幽门螺杆菌的活性。
结论:本研究表明S.cernuum的粗提物具有胃保护活性,这证实了该植物用于治疗胃溃疡的传统用途。分离的类黄酮,槲皮苷和阿夫西林以及苯丙素,异阿魏酸被认为是负责S.cernuum提取物的胃保护活性的化合物。
BACKGROUND: Solanum cernuum Vell. (Solanaceae) is a Brazilian medicinal plant, traditionally known as \"panaceia\". Its folk name is probably due to its wide range of applications in traditional medicine including the treatment of ulcers.
OBJECTIVE: To evaluate the gastroprotective activities of the hydroethanolic extract (ESC) of S. cernuum and its major isolated compounds using in vivo gastric ulcer models.
METHODS: The ESC extract was obtained by maceration followed by percolation of the dried and powdered leaves of S. cernuum in ethanol:water (7:3). The major compounds in the extract were isolated by applying various preparative chromatographic techniques. The gastroprotective activity was evaluated in mice using different gastric ulcer-induced models. The anti-Helicobacter pylori activity was performed using the agar-well diffusion and broth microdilution methods.
RESULTS: The ESC extract showed gastroprotective effects in the assay of acute gastric ulcer-induced by HCl/EtOH, nonsteroidal anti-inflammatory drug, and acetic acid-induced chronic ulcer protocols. The results also demonstrated that the gastroprotection induced by ESC extract is related to the activity of nitric oxide and endogenous sulfhydryls, which are important gastroprotective factors. The ESC extract and the alkaloid cernumidine did not show activity against H. pylori in the concentrations tested.
CONCLUSIONS: The present study showed that the crude extract of S. cernuum possessed gastroprotective activity which corroborating the traditional use of this plant for the treatment of gastric ulcers. The isolated flavonoids, quercitrin and afzelin as well as the phenylpropanoid, isoferulic acid are suggested to be the compounds responsible for the gastroprotective activity of S. cernuum extract.