促进肠道健康,现代畜牧业中普遍存在的问题,积极影响机体健康,生产力,和经济学。猪肠上皮细胞(IPEC-J2)不断增殖以维持肠道稳态,包括屏障,免疫,和吸收功能。肠道稳态是机体健康的基础。ADP-核糖基化因子1(Arf1),一个小的GTPase,在协调mTORC1对营养素的反应中起着至关重要的作用,尤其是肠道中的氨基酸。mTORC1是扩散的中心枢纽。因此,Arf1可能促进IPEC-J2细胞增殖。然而,Arf1在猪肠道中的确切作用尚不清楚。因此,我们通过Arf1在IPEC-J2细胞中的过表达和敲低,评估了Arf1在猪肠道中的功能作用和可能的机制。Arf1过表达和敲低显著增强和抑制,分别,IPEC-J2细胞活力,PCNA表达随Arf1表达而变化。此外,Arf1过表达组Ki67阳性细胞比例显著高于对照组.这些结果表明Arf1改善IPEC-J2细胞增殖。通过Western印迹探索了潜在的机制。Arf1过表达和敲低显著增强和抑制,分别,p-S6K1和p-RPS6的水平是mTORC1信号通路的关键下游靶标。总的来说,我们的发现揭示了Arf1-mTORC1轴在IPEC-J2细胞增殖中的作用及其在调节肠道稳态和健康方面的潜在功能.
The promotion of gut health, a pervasive problem in modern animal husbandry, positively affects organismal health, productivity, and economics. Porcine intestinal epithelial cells (IPEC-J2) continuously proliferate to maintain intestinal homeostasis, including barrier, immune, and absorptive functions. Gut homeostasis is fundamental to organismal health. ADP-ribosylation factor 1 (Arf1), a small GTPase, plays a crucial role in coordinating mTORC1 in response to nutrients, especially amino acid availability in the gut. mTORC1 is the central hub of proliferation. Thus, it seems likely that Arf1 promotes IPEC-J2 cell proliferation. However, the exact role of Arf1 in the porcine gut remains unclear. Therefore, we evaluated the functional role and possible mechanisms of Arf1 in the porcine intestine through Arf1 overexpression and knockdown in IPEC-J2 cells. Arf1 overexpression and knockdown significantly enhanced and inhibited, respectively, IPEC-J2 cell viability, and PCNA expression varied with Arf1 expression. Moreover, the proportion of Ki67-positive cells was significantly greater in the Arf1-overexpressing group than in the control group. These results suggest that Arf1 improves IPEC-J2 cell proliferation. The underlying mechanism was explored by Western blotting. Arf1 overexpression and knockdown significantly enhanced and suppressed, respectively, the levels of p-S6K1 and p-RPS6, which are key downstream targets of the mTORC1 signaling pathway. Collectively, our findings reveal the role of the Arf1-mTORC1 axis in IPEC-J2 cell proliferation and its potential function in regulating intestinal homeostasis and health.