背景:研究表明,衰老之间存在显著的相关性,免疫微环境,炎症和肿瘤。然而,衰老之间的关系,免疫微环境,膀胱膀胱炎和膀胱尿路上皮癌(BLCA)的报道很少。
方法:将年轻和老年小鼠的膀胱单细胞和转录组数据用于免疫景观分析。转录组,使用BLCA和间质性膀胱炎/膀胱疼痛综合征(IC/BPS)的单细胞和癌症基因组图谱程序数据集来分析免疫细胞浸润和分子表达。来自小鼠的膀胱组织,收集IC/BPS和BLCA以验证结果。
结果:八种类型的免疫细胞(巨噬细胞,B细胞,树突状细胞,T细胞,单核细胞,自然杀伤细胞,在小鼠膀胱中鉴定出γδT细胞和ILC2)。老年小鼠膀胱组织的T细胞数量明显更高,γδT细胞,ILC2和B细胞高于青年组(P<0.05)。三种类型的T细胞(NKT细胞,γδT细胞和幼稚T细胞)和三种类型的B细胞(滤泡B细胞,血浆和记忆B细胞)在老年小鼠膀胱中鉴定。趋化因子受体7(CCR7)在老年膀胱中高表达,IC/BPS和BLCA(P<0.05)。CCR7可能参与老年膀胱T细胞和B细胞浸润,IC/BPS和BLCA。有趣的是,CCR7在BLCA细胞膜上的高表达是预后保护因素.
结论:在这项研究中,我们表征了免疫细胞在老年和年轻小鼠膀胱组织中的表达谱,并证明CCR7介导的T细胞和B细胞过滤有助于膀胱衰老的发展。IC/BPS和BLCA。
BACKGROUND: Research has suggested significant correlations among ageing, immune microenvironment, inflammation and tumours. However, the relationships among ageing, immune microenvironment, cystitis and bladder urothelial carcinoma (BLCA) in the bladder have rarely been reported.
METHODS: Bladder single-cell and transcriptomic data from young and old mice were used for immune landscape analysis. Transcriptome, single-cell and The Cancer Genome Atlas Program datasets of BLCA and interstitial cystitis/bladder pain syndrome (IC/BPS) were used to analyse immune cell infiltration and molecular expression. Bladder tissues from mice, IC/BPS and BLCA were collected to validate the results.
RESULTS: Eight types of immune cells (macrophages, B-cells, dendritic cells, T-cells, monocytes, natural killer cells, γδ T-cells and ILC2) were identified in the bladder of mice. Aged mice bladder tissues had a significantly higher number of T-cells, γδ T-cells, ILC2 and B-cells than those in the young group (P < 0.05). Three types of T-cells (NK T-cells, γδ T-cells and naïve T-cells) and three types of B-cells (follicular B-cells, plasma and memory B-cells) were identified in aged mice bladder. Chemokine receptor 7 (CCR7) is highly expressed in aged bladder, IC/BPS and BLCA (P < 0.05). CCR7 is likely to be involved in T- and B-cell infiltration in aged bladder, IC/BPS and BLCA. Interestingly, the high CCR7 expression on BLCA cell membranes was a prognostic protective factor.
CONCLUSIONS: In this study, we characterised the expression profiles of immune cells in bladder tissues of aged and young mice and demonstrated that CCR7-mediated T- and B-cell filtration contributes to the development of bladder ageing, IC/BPS and BLCA.