■多巴胺,一种常用于危重病人的治疗剂,最近被证明与临床感染有关,然而,这种关联背后的确切机制仍然难以捉摸。拟气化克雷伯菌,一种属于克雷伯氏菌属的新菌株,表现出潜在的致病属性。多巴胺对K.quasivariicola感染的影响引起了我们的兴趣。
■考虑到感染过程中宿主免疫因素的作用,这项研究旨在调查K.quasivariicola之间的复杂相互作用,多巴胺,和巨噬细胞被探索。
■RAW264.7细胞和C57/BL6小鼠被K.quasivariicola感染,巨噬细胞内的细菌生长,检测小鼠肺部炎症细胞因子的产生和病理变化,在不存在或存在多巴胺的情况下。
■多巴胺抑制了K.quasivariicola在培养基中的生长,但与巨噬细胞共培养时促进细菌生长。K.quasivariicola感染的RAW264.7细胞中促炎细胞因子的表达增加,并且在添加多巴胺后观察到显着升高。K.quasivariicola感染小鼠引起炎症反应和肺损伤,多巴胺的使用加剧了这种情况。
■我们的研究结果表明,多巴胺可能是与K.quasivalicola感染相关的潜在危险因素之一。这一经验见解为临床精准医疗提供了坚实的参考。此外,提出了一种用于抑制剂筛选的微生物-药物-宿主免疫细胞的体外模型,以更准确地复制复杂的体内环境。这项基本工作有助于目前对病原体之间串扰的理解,多巴胺和宿主免疫细胞。
UNASSIGNED: Dopamine, a frequently used therapeutic agent for critically ill patients, has been shown to be implicated in clinical infections recently, however, the precise mechanisms underlying this association remain elusive. Klebsiella quasivariicola, a novel strain belonging to the Klebsiella species, exhibits potential pathogenic attributes. The impact of dopamine on K. quasivariicola infection has aroused our interest.
UNASSIGNED: Considering the contribution of host immune factors during infection, this study aimed to investigate the intricate interactions between K. quasivariicola, dopamine, and macrophages were explored.
UNASSIGNED: RAW264.7 cells and C57/BL6 mice were infected with K. quasivariicola, and the bacterial growth within macrophage, the production of inflammatory cytokines and the pathological changes in mice lungs were detected, in the absence or presence of dopamine.
UNASSIGNED: Dopamine inhibited the growth of K. quasivariicola in the medium, but promoted bacterial growth when co-cultured with macrophages. The expression of proinflammatory cytokines increased in RAW 264.7 cells infected with K. quasivariicola, and a significant rise was observed upon the addition of dopamine. The infection of K. quasivariicola in mice induced an inflammatory response and lung injury, which were exacerbated by the administration of dopamine.
UNASSIGNED: Our findings suggest that dopamine may be one of the potential risk factors associated with K. quasivariicola infection. This empirical insight provides solid references for clinical precision medicine. Furthermore, an in vitro model of microbes-drugs-host immune cells for inhibitor screening was proposed to more accurately replicate the complex in vivo environment. This fundamental work had contributed to the present understanding of the crosstalk between pathogen, dopamine and host immune cells.