■辣椒脉斑驳病毒(PVMV)属于Potyviridae家族中的Potyvirus属,是辣椒生产的主要威胁,导致产量和果实质量下降;然而,缺乏有效的农药和化学处理方法来保护植物免受病毒感染。因此,迫切需要发现源自天然来源的高活性和环境友好的抗病毒剂。芽孢杆菌。被广泛用作控制真菌的生物防治剂,细菌,和病毒性植物病害。特别是,Velezensis芽孢杆菌HN-2对植物病原体具有很强的抗生素活性,并且还可以诱导植物抗性。
■本研究中使用的实验对象是VelezensisHN-2,苯并噻二唑,还有杜富林,旨在评估它们对抗氧化活性的影响,活性氧的水平,防御酶的活性,和防御相关基因在烟草中的表达。此外,研究了巴西芽孢杆菌HN-2在辣椒中的定殖能力。
■生物测定的结果表明,VelezensisHN-2芽孢杆菌具有强大的定植能力,特别是在细胞间隙中,通过多种植物防御机制导致延迟感染和增强对PVMV的保护,从而促进植物生长。此外,VelezensisHN-2增加了抗氧化酶的活性,从而减轻本氏烟草中PVMV诱导的ROS产生。此外,在5dpi应用VelezensisHN-2显着增加了JA响应基因的表达,而水杨酸反应基因的表达保持不变,暗示JA信号通路的激活是Velezensis芽孢杆菌HN-2诱导的抗PVMV活性的关键机制。免疫印迹分析显示,HN-2治疗在15dpi时延迟PVMV感染,进一步突出其在诱导植物抗性和促进生长发育中的作用。
■这些发现强调了VelezensisHN-2在有效管理病毒性植物病害中的田间应用的潜力。
UNASSIGNED: Pepper veinal mottle virus (PVMV) belongs to the genus Potyvirus within the family Potyviridae and is a major threat to pepper production, causing reduction in yield and fruit quality; however, efficient pesticides and chemical treatments for plant protection against viral infections are lacking. Hence, there is a critical need to discover highly active and environment-friendly antiviral agents derived from natural sources. Bacillus spp. are widely utilized as biocontrol agents to manage fungal, bacterial, and viral plant diseases. Particularly, Bacillus velezensis HN-2 exhibits a strong antibiotic activity against plant pathogens and can also induce plant resistance.
UNASSIGNED: The experimental subjects employed in this study were Bacillus velezensis HN-2, benzothiadiazole, and dufulin, aiming to evaluate their impact on antioxidant activity, levels of reactive oxygen species, activity of defense enzymes, and expression of defense-related genes in Nicotiana benthamiana. Furthermore, the colonization ability of Bacillus velezensis HN-2 in Capsicum chinense was investigated.
UNASSIGNED: The results of bioassays revealed the robust colonization capability of Bacillus velezensis HN-2, particularly in intercellular spaces, leading to delayed infection and enhanced protection against PVMV through multiple plant defense mechanisms, thereby promoting plant growth. Furthermore, Bacillus velezensis HN-2 increased the activities of antioxidant enzymes, thereby mitigating the PVMV-induced ROS production in Nicotiana benthamiana. Moreover, the application of Bacillus velezensis HN-2 at 5 dpi significantly increased the expression of JA-responsive genes, whereas the expression of salicylic acid-responsive genes remained unchanged, implying the activation of the JA signaling pathway as a crucial mechanism underlying Bacillus velezensis HN-2-induced anti-PVMV activity. Immunoblot analysis revealed that HN-2 treatment delayed PVMV infection at 15 dpi, further highlighting its role in inducing plant resistance and promoting growth and development.
UNASSIGNED: These findings underscore the potential of Bacillus velezensis HN-2 for field application in managing viral plant diseases effectively.