动物从其环境中吸收氧气的最大速率(O2,max)是其生理学和生态学的关键方面。在鱼类中,通常通过在增量游泳测试或从穷举追逐中恢复过程中测量摄氧量来量化O2,max。在这篇评论中,我们汇编了最近的研究,将这两种技术应用于同一条鱼,并表明这两种方法通常对一组个体产生不同的O2,max的平均估计值。此外,在一群鱼里,游泳过程中确定的心氧最大值估计值与追逐恢复过程中确定的估计值相关性差(即个体的心氧最大值在不同方法中不可重复)。缺乏共识的一种解释是这些方法测量不同的生理状态,每个人都有自己的行为,解剖和生化决定因素。我们建议这些方法不能直接互换,但是,更确切地说,每个都适合解决鱼类生物学中的不同问题。我们建议研究人员选择反映其研究生物学背景的方法,并且我们主张使用准确的术语来承认用于升高血管O2的技术(例如,峰值血管O2,游泳或峰值血管O2,恢复)。如果研究的目的是估计\'真实\'O2,最大的个体或物种,我们建议试点研究比较方法,最好使用重复措施设计。我们希望这些建议能够为鱼类内部和之间的O2,max变化的原因和后果提供新的见解。
The maximum rate at which animals take up oxygen from their environment (ṀO2,max) is a crucial aspect of their physiology and ecology. In fishes, ṀO2,max is commonly quantified by measuring oxygen uptake either during incremental swimming tests or during recovery from an exhaustive chase. In this Commentary, we compile recent studies that apply both techniques to the same fish and show that the two methods typically yield different mean estimates of ṀO2,max for a group of individuals. Furthermore, within a group of fish, estimates of ṀO2,max determined during swimming are poorly correlated with estimates determined during recovery from chasing (i.e. an individual\'s ṀO2,max is not repeatable across methods). One explanation for the lack of agreement is that these methods measure different physiological states, each with their own behavioural, anatomical and biochemical determinants. We propose that these methods are not directly interchangeable but, rather, each is suited to address different questions in fish biology. We suggest that researchers select the method that reflects the biological contexts of their study, and we advocate for the use of accurate terminology that acknowledges the technique used to elevate ṀO2 (e.g. peak ṀO2,swim or peak ṀO2,recovery). If the study\'s objective is to estimate the \'true\' ṀO2,max of an individual or species, we recommend that pilot studies compare methods, preferably using repeated-measures designs. We hope that these recommendations contribute new insights into the causes and consequences of variation in ṀO2,max within and among fish species.