日益增长的环境问题促使人们努力通过采用可生物降解的聚合物来减少包装废物,涂层,和电影。然而,用于包装的可生物降解材料面临与阻隔性能相关的挑战,机械强度,和处理兼容性。使用可生物降解的化合物(醇溶蛋白,d-甘露糖,柠檬酸),作为涂料来增加食品包装材料的氧气屏障。为了提高凝胶稳定性和机械性能,凝胶与原硅酸四乙酯和原硅酸四甲酯前体合成的颗粒物理交联。此外,对人类角质形成细胞和成纤维细胞进行生物相容性评估,证明了用于消费者接触的凝胶的安全性。对凝胶特性进行了表征,包括分子结构,形态学,和地形。使用生物发光ATP测定法评估凝胶的生物相容性以检测细胞活力。乳酸脱氢酶测定细胞毒性,和白细胞刺激测试以检测炎症潜能。制备了在低湿度环境下具有强氧阻隔性能的复合凝胶。在凝胶制备期间将硅烷前体增加到50重量%减缓了在水中的降解。添加柠檬酸降低了凝胶溶解度。然而,更高的前体数量增加了表面粗糙度,使凝胶更脆但机械抗性。凝胶中前体的增加也增加了凝胶粘度。重要的是,凝胶对人角质形成细胞或成纤维细胞无细胞毒性,对白细胞无炎症作用。这种复合凝胶有望用于氧气阻隔食品包装,并且对消费者接触是安全的。进一步的研究应集中在优化氧气屏障在潮湿环境中的稳定性,并研究可生物降解材料对消费者的潜在敏化作用。
Growing environmental concerns drive efforts to reduce packaging waste by adopting biodegradable polymers, coatings, and films. However, biodegradable materials used in packaging face challenges related to barrier properties, mechanical strength, and processing compatibility. A composite gel was developed using biodegradable compounds (prolamin, d-mannose, citric acid), as a coating to increase the oxygen barrier of food packaging materials. To improve gel stability and mechanical properties, the gels were physically cross-linked with particles synthesized from tetraethyl orthosilicate and tetramethyl orthosilicate precursors. Additionally, biocompatibility assessments were performed on human keratinocytes and fibroblasts, demonstrating the safety of the gels for consumer contact. The gel properties were characterized, including molecular structure, morphology, and topography. Biocompatibility of the gels was assessed using bioluminescent ATP assay to detect cell viability, lactate dehydrogenase assay to determine cell cytotoxicity, and a leukocyte stimulation test to detect inflammatory potential. A composite gel with strong oxygen barrier properties in low-humidity environments was prepared. Increasing the silane precursor to 50 wt% during gel preparation slowed degradation in water. The addition of citric acid decreased gel solubility. However, higher precursor amounts increased surface roughness, making the gel more brittle yet mechanically resistant. The increase of precursor in the gel also increased gel viscosity. Importantly, the gels showed no cytotoxicity on human keratinocytes or fibroblasts and had no inflammatory effects on leukocytes. This composite gel holds promise for oxygen barrier food packaging and is safe for consumer contact. Further research should focus on optimizing the stability of the oxygen barrier in humid environments and investigate the potential sensitizing effects of biodegradable materials on consumers.