背景:鳞状细胞癌是最常见的生殖器,马眼和胃肿瘤。在马阴茎鳞状细胞癌(SCC)和前体病变的多项研究中,已检测到马乳头瘤病毒2型(EcPV2)DNA,这提供了EcPV2在马生殖器SCC中的因果作用的证据。最近,EcPV2E6/E7核酸也在马胃SCC中检测到,但需要进一步的研究来确定EcPV2感染在胃SCC发病机制中的作用。EcPV2核酸在眼部SCC和前体病变中很少被描述。
目的:通过聚合酶链反应(PCR)和原位杂交(ISH)研究阴茎增生中EcPV2核酸的存在,马的乳头状瘤和SCC,并确定是否可以在影响其他位置的SCC中检测到EcPV2核酸,包括胃,眼组织和喉。
方法:21份福尔马林固定石蜡包埋(FFPE)组织样本,包括12个生殖器病变,包括阴茎增生,乳头状瘤和SCC,6眼SCC,2例胃SCC和1例喉SCC,通过PCR和ISH筛选EcPV2E6/E7的DNA和mRNA。包括来自六匹马的档案FFPE组织样本(眼睑和阴茎粘膜和包皮),而没有肿瘤或乳头瘤病毒相关疾病的诊断或病史。
结果:通过PCR和ISH在所有生殖器病变(12/12)和胃SCCs(2/2)中检测到EcPV2核酸,在两个眼部SCC(2/6)和一个喉部SCC(1/1)中。在控制马匹中,一个眼睑样本在PCR中呈阳性,但在ISH中无阳性.剩余的对照样品在PCR和ISH中对于EcPV2E6/E7核酸是阴性的。
结论:这些结果进一步支持了EcPV2感染在马生殖器SCC发展中的作用,并表明EcPV2感染也可能是马其他SCC的诱发因素。包括胃,眼和喉部SCC。
Squamous cell carcinoma is the most common genital, ocular and gastric tumour in horses. Equus caballus papillomavirus type 2 (EcPV2) DNA has been detected in several studies in equine penile squamous cell carcinomas (SCCs) and precursor lesions providing evidence of a causal role of EcPV2 in equine genital SCCs. Recently, EcPV2 E6/E7 nucleic acids were also detected in equine gastric SCCs, but further studies are required to determine the role of EcPV2 infection in the pathogenesis of gastric SCC. EcPV2 nucleic acids have been rarely described in ocular SCCs and precursor lesions.
To investigate the presence of EcPV2 nucleic acids with polymerase chain reaction (PCR) and in situ hybridisation (ISH) in penile hyperplasias, papillomas and SCCs in horses and to determine whether EcPV2 nucleic acids can be detected in SCCs affecting other locations, including the stomach, ocular tissues and larynx.
Twenty-one archival formalin-fixed paraffin embedded (FFPE) tissue samples, including 12 genital lesions comprising penile hyperplasias, papillomas and SCCs, 6 ocular SCCs, 2 gastric SCCs and 1 laryngeal SCC, were screened by PCR and ISH for EcPV2 E6/E7 DNA and mRNA. Archival FFPE tissue samples (eyelid and penile mucosa and preputium) from six horses without a diagnosis or history of neoplastic or papillomavirus-associated disease were included as controls.
EcPV2 nucleic acids were detected by PCR and ISH in all genital lesions (12/12) and gastric SCCs (2/2), in two ocular SCCs (2/6) and in one laryngeal SCC (1/1). In control horses, one eyelid sample was positive in PCR but not in ISH. The remaining control samples were negative for EcPV2 E6/E7 nucleic acids in PCR and ISH.
These results further support the role of EcPV2 infection in the development of equine genital SCCs and suggest that EcPV2 infection may also act as a predisposing factor for other SCCs in horses, including gastric, ocular and laryngeal SCCs.