近年来,Helichrysumitalicum引起了许多研究人员的兴趣,主要是它的精油,但它的多酚含量也越来越高。在目前的研究中,我们研究了在保加利亚生长的意大利红藻的多酚组成。多酚络合物用各种极性的溶剂分馏,包括己烷,氯仿,乙酸乙酯,和丁醇,以评估成分的生物学影响。HPLC-PDA和UHPLC-MS/MS用于检查所有级分。在多酚成分检测方法中,绿咖啡指纹图谱被用作“替代标准”。从UHPLC-MS/MS分析,我们确定了60种成分的多酚复合物,如槲皮素3-O-葡糖苷酸,槲皮素乙酰糖苷,异鼠李素乙酰糖苷,异鼠李素咖啡酰糖苷,槲皮素咖啡酰-丙二酰-糖苷,异鼠李素香豆酰基糖苷,香豆酰基-咖啡酰基奎宁酸,和diCQA-乙酰基-衍生物首次报道在意大利赤霉菌的组合物中。在体外和计算机上评估了馏分的生物活性,其中包括对抗氧化应激(过氧化氢清除活性(HPSA),羟自由基清除活性(HRSA),金属螯合活性(MChA))和亚硝基(一氧化氮清除活性)(NOSA),体外抗炎,和抗关节炎活性。结果表示为IC50±SDμg/mL。分析显示,EtOAc级分的特征在于最高的HPSA(57.12±1.14μg/mL),HRSA(92.23±1.10μg/mL),MChA(5.60±0.17μg/mL),和NOSA(89.81±2.09μg/mL),而己烷和氯仿级分显示出明显更高的体外抗炎活性(30.48±2.33μg/mL,62.50±1.69μg/mL)与标准布洛芬相比。所有三个部分均显示出潜在的抗关节炎活性(102.93±8.62μg/mL,108.92±4.42μg/mL,84.19±3.89μg/mL)。
Helichrysum italicum has piqued the interest of many researchers in recent years, mostly for its essential oil, but increasingly for its polyphenolic content as well. In the current study, we examine the polyphenolic composition of H. italicum grown in Bulgaria. The polyphenolic complex was fractionated with solvents of various polarities, including hexane, chloroform, ethyl acetate, and butanol, in order to assess the biological impact of the components. HPLC-PDA and UHPLC-MS/MS were used to examine all fractions. The green coffee fingerprint profile was employed as a \"surrogate standard\" in the polyphenolic components detection approach. From the UHPLC-MS/MS analysis, we identified 60 components of the polyphenolic complex such as quercetin 3-O-glucuronide, quercetin acetyl-glycoside, isorhamnetin acetyl-glycoside, isorhamnetin caffeoyl-glycoside, quercetin caffeoyl-malonyl-glycoside, isorhamnetin coumaroyl-glycoside, coumaroyl-caffeoylquinic acid, and diCQA-acetyl-derivative were first reported in the composition of H. italicum. The biological activity of the fractions was evaluated in vitro and in silico, which included the fight against oxidative stress (hydrogen peroxide scavenging activity (HPSA), hydroxyl radical scavenging activity (HRSA), metal-chelating activity (MChA)) and nitrosative (nitric oxide scavenging activity) (NOSA)), in vitro anti-inflammatory, and anti-arthritic activity. Results are presented as IC50 ± SD μg/mL. The analysis showed that the EtOAc fraction was characterized by highest HPSA (57.12 ± 1.14 μg/mL), HRSA (92.23 ± 1.10 μg/mL), MChA (5.60 ± 0.17 μg/mL), and NOSA (89.81 ± 2.09 μg/mL), while the hexane and chloroform fractions showed significantly higher in vitro anti-inflammatory activity (30.48 ± 2.33 μg/mL, 62.50 ± 1.69 μg/mL) compared to the standard ibuprofen. All three fractions showed potential anti-arthritic activity (102.93 ± 8.62 μg/mL, 108.92 ± 4.42 μg/mL, 84.19 ± 3.89 μg/mL).