锐波波纹(SWR)振荡在非快速眼动睡眠期间的记忆巩固中起关键作用,不动,和完善的行为。然而,时间调制的突触兴奋或抑制是波纹的基础是有争议的。为了解决这个问题,我们同时记录了清醒小鼠体内CA1区的兴奋性和抑制性突触后电流(EPSC和IPSC)和局部场电位(LFP).在SWR期间,抑制高于激励,峰值电导比为4.1±0.5。此外,SWR相关的IPSCs的振幅与SWR大小呈正相关,而EPSCs则没有。最后,相位分析表明,IPSC被锁相到单个纹波周期,而EPSC在相空间中均匀分布。光遗传学抑制表明PV中间神经元对SWR相关的IPSC提供了主要贡献。因此,阶段性抑制,但不是激励,在体内塑造海马CA1区的SWR振荡。
Sharp wave-ripple (SWR) oscillations play a key role in memory consolidation during non-rapid eye movement sleep, immobility, and consummatory behavior. However, whether temporally modulated synaptic excitation or inhibition underlies the ripples is controversial. To address this question, we performed simultaneous recordings of excitatory and inhibitory postsynaptic currents (EPSCs and IPSCs) and local field potentials (LFPs) in the CA1 region of awake mice in vivo. During SWRs, inhibition dominated over excitation, with a peak conductance ratio of 4.1 ± 0.5. Furthermore, the amplitude of SWR-associated IPSCs was positively correlated with SWR magnitude, whereas that of EPSCs was not. Finally, phase analysis indicated that IPSCs were phase-locked to individual ripple cycles, whereas EPSCs were uniformly distributed in phase space. Optogenetic inhibition indicated that PV+ interneurons provided a major contribution to SWR-associated IPSCs. Thus, phasic inhibition, but not excitation, shapes SWR oscillations in the hippocampal CA1 region in vivo.