目的:确定适当的时间点开始定期运动,以减少与年龄有关的焦虑和社交行为受损。
方法:对于本研究,8周龄雄性Wistar大鼠分为三组:无运动(NoEX),短期锻炼(S-Ex),和长期运动(L-Ex)组。S-Ex组大鼠在12个月大时开始跑步机运动,而L-Ex大鼠从2月龄开始。运动大鼠被迫每周在跑步机上行走三次,在前2周内以1至2天的间隔为10分钟,10米/分钟,直到17个月大,和8米/分钟后。在19个月大的时候,进行行为测试以评估运动对年龄诱发的行为改变的影响,并进行定量聚合酶链反应以揭示行为改变背后的机制.
结果:长期运动改善了焦虑样行为。此外,属于S-Ex和L-Ex组的大鼠表现出改善的社交行为和对有趣物体的好奇心。qPCR数据显示,跑步机运动抑制了老年大鼠前额叶皮层中立即早期基因的表达。
结论:这项研究表明,长期运动抑制早期反应基因,以这种方式,它增加了对压力的抵抗力,减少焦虑相关行为,改善社会行为。这些发现强调需要考虑适当的时间开始运动,以防止压力引起的焦虑相关行为。
OBJECTIVE: To determine the appropriate time points to start regular exercise which could reduce age-related anxiety and impaired social behavior.
METHODS: For this study, 8-week-old male Wistar rats were divided into three groups: no exercise (NoEX), short-term exercise (S-Ex), and long-term exercise (L-Ex) groups. S-Ex-group rats started treadmill exercise at 12 months of age, while L-Ex rats started from at 2 months of age. Exercise rats were forced to walk on the treadmill three times per week, with 1- to 2-day intervals for 10 minutes during the first 2 weeks, at 10 m/min until 17 months of age, and at 8 m/min thereafter. At 19 months of age, behavioral tests were performed to assess the effects of exercise on age-induced behavioral change as well as quantitative polymerase chain reaction were done to uncover the mechanism behind the behavioral changes.
RESULTS: Anxiety-like behavior was improved by long-term exercise. Additionally, rats belonging to the S-Ex and L-Ex groups showed improved social behavior and increased curiosity about interesting objects. The qPCR data showed that treadmill exercise suppressed the expression of immediate-early genes in the prefrontal cortex of the aged rats.
CONCLUSIONS: This study suggests that long-term exercise represses early response genes, and in this way, it increases resistance to stress, diminishes anxiety-related behavior, and improves social behavior. These findings underscore the need to consider appropriate time to start exercise to prevent stress induced anxiety related behavior.