背景:高热,痛觉和炎症是用合成和草药治疗的病理性疾病。本研究的基本目的是通过使用体内和计算机研究来评估C.colocynthis(水果提取物)中存在的植物成分的药理活性。
方法:我们的研究中使用了36只白化病大鼠,平均体重在150-170g之间。使用角叉菜胶(来自红海藻的提取物)研究了抗炎活性,所述角叉菜胶在白化病大鼠爪子中诱导水肿。然而,在解热和镇痛活性研究中,酵母和乙酸用于引起发热或痛觉,分别。不同剂量的丙酮果实提取物用于治疗炎症,发热和痛觉。
结果:我们的结果表明,丙酮酸果实提取物在抗炎和镇痛活性中的最大抑制百分比分别为70%和100%,分别,400毫克/千克的剂量,在100mg/kg剂量下,最大抑制百分比为86%。在硅分析中,我们已经证明了生物活性化合物(α-spinasterol,在水果提取物中发现的抗坏血酸和绿原酸)具有突出的抑制特性,涉及蛋白质PTGS2,TLR2和TRPV4。C.colocynthis果实提取物显示出统计学上显著的结果(p<0.005)并且与参考药物相当。C.colocynthis的丙酮果实提取物可用作天然和安全的补救措施,无副作用。
结论:在绿原酸的计算机模拟研究中,抗坏血酸和α-脊椎甾醇已显示这些是可用于增强免疫应答的抑制性化合物。
BACKGROUND: Hyperpyrexia, algesia and inflammation are pathological disorders which are treated with synthetic as well as herbal medications.
OBJECTIVE: The basic aim of the present study is to evaluate the ethnopharmacological activities of phytoconstituents that are present in C. colocynthis (fruit extract) by using in vivo and in silico studies.
METHODS: Thirty-six albino rats were used in our studies with an average weight between 150-170 g. Anti-inflammatory activity was investigated using carrageenan (an extract from a red seaweed) that induced edema in albino rat paws. However, in antipyretic and analgesic activity studies, yeast and acetic acid were used to cause pyrexia or algesia, respectively. Different doses of acetone fruit extract were used to treat inflammation, pyrexia and algesia.
RESULTS: Our results showed that the maximum percentage inhibition of acetonic fruit extract in anti-inflammatory and analgesic activities was observed at 70% and 100%, respectively, with 400 mg/kg doses, and in pyretic activity the maximum inhibitory percentage was 86% with a 100 mg/kg dose. In in silico analysis, we have shown that bioactive compounds (α-spinasterol, ascorbic acid and chlorogenic acid) found in fruit extract have outstanding inhibition properties that involves proteins PTGS2, TLR2 and TRPV4. C. colocynthis fruit extract shows results that are statistically significant (p < 0.005) and comparable to a reference drug. Acetonic fruit extract of C. colocynthis can be used as a natural and safe remedy with no side effects.
CONCLUSIONS: Both in vivo and in silico studies on chlorogenic acid, ascorbic acid and α-spinasterol have shown that these are inhibitory compounds that can be used for boosting the immune response.