背景:高血糖状况与更严重的牙周炎和非手术牙周治疗(NPT)后较差的预后相关。然后,这些患者是NPT相关辅助治疗的候选人.这项研究评估了不同波长的光生物调节(PBMT)对非高血糖/高血糖动物牙周修复的影响。
方法:64只大鼠接受结扎法诱导牙周炎。这些动物中有一半诱发高血糖,而另一半仍然是非高血糖。根据结扎时应用的PBMT方案将动物细分为4组(n=8):CTR:无PBMT;IRL:具有红外激光(808nm)的PBMT;RL:具有红色激光(660nm)的PBMT;和RL-IRL:具有红色(660nm)和红外激光(808nm)的PBMT。经过7天的时间,动物被安乐死。通过显微断层扫描评估的参数是相对于总组织体积的骨体积(BV/TV%),从牙骨质-釉质交界处到骨顶部的距离(CEJ-CB),小梁厚度,小梁之间的空间,和小梁的数量。此外,炎症细胞的百分比,血管,和结缔组织基质通过组织形态学分析进行评估。
结果:PBMT减少了高血糖动物的骨丢失并增加了小梁密度(p<0.05),RL更有效地减少线性骨丢失(CEJ-CB),而RL-IRL在维持BV/TV%方面更有效。PBMT在高血糖动物中减少血管并增加结缔组织成分(p<0.05)。不管动物的全身状况如何,RL-IRL减少炎性细胞(p<.05)。
结论:PBMT(RL,RL-IRL)改善高血糖动物牙周组织的修复。
BACKGROUND: Hyperglycemic conditions is associated with more severe periodontitis and poorer outcomes after nonsurgical periodontal treatment (NPT). Then, these patients are candidates for adjunctive therapy associated with NPT. This study evaluates the effect of photobiomodulation (PBMT) at different wavelengths on periodontal repair in non-hyperglycemic/hyperglycemic animals.
METHODS: Sixty-four rats were submitted to induction of periodontitis by ligatures. Hyperglycemia was induced in half of these animals, whereas the other half remained non-hyperglycemic. The animals were subdivided into 4 groups according to the PBMT protocol applied at the time of ligature removal (n = 8): CTR: Without PBMT; IRL: PBMT with infrared laser (808 nm); RL: PBMT with red laser (660 nm); and RL-IRL: PBMT with red (660 nm) and infrared laser (808 nm). After a period of 7 days, the animals were euthanized. The parameters assessed by microtomography were the bone volume relative to total tissue volume (BV/TV%), distance from the cemento-enamel junction to the top of the bone crest (CEJ-CB), trabecular thickness, space between trabeculae, and number of trabeculae. Additionally, the percentage of inflammatory cells, blood vessels, and connective tissue matrix were assessed by histomorphometric analysis.
RESULTS: PBMT reduced bone loss and increased trabecular density in hyperglycemic animals (p < .05), with RL being more effective in reducing linear bone loss (CEJ-CB), whereas RL-IRL was more effective in maintaining BV/TV%. PBMT reduced blood vessels and increased the connective tissue component in hyperglycemic animals (p < .05). RL-IRL reduced inflammatory cells regardless of the systemic condition of the animal (p < .05).
CONCLUSIONS: PBMT (RL, RL-IRL) improves the repair of periodontal tissues in hyperglycemic animals.