■本研究旨在研究温水浸泡(WWI)对炎症的影响,肾功能,糖尿病(DM)大鼠肾脏组织损伤。
■将40只雄性大鼠分为四组:健康对照(HC),糖尿病控制(DC),用WWI(DW)治疗的糖尿病大鼠,和用WWI(HW)治疗的健康大鼠。每天在43°C下进行15分钟的WWI会话,持续八周。各种参数,包括脂质,空腹血糖(FBS),HbA1C,胰岛素,糖基化终产物(AGEs),HSP70,肾小球滤过率(GFR),尿白蛋白排泄,肌酐,血尿素氮(BUN),氧化应激,抗氧化剂参数,和RAGE的基因表达,VEGF,和TGFβ1进行了评估。还进行了肾组织的组织学检查。
■FBS显著降低,AGEs,谷胱甘肽,超氧化物歧化酶(SOD),与DC相比,DW组观察到一氧化氮(NO)水平。RAGE的表达,VEGF,TGFβ1基因在DW中下降。甘油三酯,总胆固醇,DW中LDL胆固醇较低。胰岛素,HDL胆固醇,过氧化氢酶,总抗氧化能力(TAC),DW组织HSP70较高。组织学评估显示,与DC相比,DW中的肾脏损伤减少。
■WWI八周显示出减轻大鼠糖尿病肾病的希望,提示其作为治疗糖尿病并发症的非侵入性辅助治疗的潜力。
UNASSIGNED: This study aimed to investigate the effects of Warm Water Immersion (WWI) on inflammation, kidney function, and kidney tissue damage in rats with diabetes mellitus (DM).
UNASSIGNED: Forty male rats were divided into four groups: Healthy Control (HC), Diabetic Control (DC), Diabetic Rats treated with WWI (DW), and Healthy Rats treated with WWI (HW). Daily 15-minute WWI sessions at 43 °C were administered for eight weeks. Various parameters including lipids, fasting blood sugar (FBS), HbA1C, insulin, advanced glycation end products (AGEs), HSP70, glomerular filtration rate (GFR), urinary albumin excretion, creatinine, blood urea nitrogen (BUN), oxidative stress, anti-oxidant parameters, and gene expression of RAGE, VEGF, and TGFß1 were assessed. Histological examination of kidney tissue was also conducted.
UNASSIGNED: Significant reductions in FBS, AGEs, glutathione, superoxide dismutase (SOD), and nitric oxide (NO) levels were observed in the DW group compared to DC. Expression of RAGE, VEGF, and TGFß1 genes decreased in DW. Triglycerides, total cholesterol, and LDL cholesterol were lower in DW. Insulin, HDL cholesterol, catalase, total anti-oxidant capacity (TAC), and tissue HSP70 were higher in DW. Histological assessment revealed reduced kidney damage in DW compared to DC.
UNASSIGNED: WWI for eight weeks shows promise in mitigating diabetic nephropathy in rats, suggesting its potential as a non-invasive adjunctive therapy for managing diabetes complications.