温泉中居住着特定的微生物群落,这些微生物群落是新型分类群的水库。在这项工作中,菌株4228-RoLT从Solnechny温泉中分离出来,乌松·卡尔德拉,堪察加半岛.菌株4228-RoLT的细胞是形成多细胞丝的革兰氏阴性棒。该菌株在60°C和pH7.0下最佳生长,并发酵了各种有机化合物,包括多糖(微晶纤维素,木聚糖,甲壳素,淀粉,糊精,葡聚糖,β-葡聚糖,半乳甘露聚糖,葡甘露聚糖,甘露聚糖)。主要脂肪酸是异-C17:0、C16:0、C18:0、C20:0、异-C19:0、前-C17:0和C22:0。该菌株的基因组为3.25Mbp,GC含量为54.2%。根据全基因组比较和系统基因组分析,新的分离株隶属于绿藻门的Anaerolineae类内的Thermanaerothrix属的一种新物种,名字T.solarissp.11月。以4228-RoLT(=VKMB-3776T=UQM41594T=BIMB-2058T)为类型菌株。发现在菌株4228-RoLT的基因组中编码114个CAZymes,包括43个糖苷水解酶。菌株4228-RoLT的细胞结合酶和胞外酶对淀粉具有活性,葡聚糖,甘露聚糖,木聚糖和各种纤维素,对β-葡聚糖的活性最高。总之,生长实验,酶活性测定和基因组分析表明,T.solaris菌株4228-RoLT可以作为适合植物生物质水解的糖苷酶的来源。
Hot springs are inhabited by specific microbial communities which are reservoirs of novel taxa. In this work strain 4228-RoLT was isolated from the Solnechny hot spring, Uzon Caldera, Kamchatka. Cells of the strain 4228-RoLT were Gram-negative rods forming multicellular filaments. The strain grew optimally at 60 °C and pH 7.0 and fermented various organic compounds including polysaccharides (microcrystalline cellulose, xylan, chitin, starch, dextrin, dextran, beta-glucan, galactomannan, glucomannan, mannan). Major fatty acids were iso-C17:0, C16:0, C18:0, C20:0, iso-C19:0, anteiso-C17:0 and C22:0. Genome of the strain was of 3.25 Mbp with GC content of 54.2%. Based on the whole genome comparisons and phylogenomic analysis the new isolate was affiliated to a novel species of Thermanaerothrix genus within Anaerolineae class of phylum Chloroflexota, for which the name T. solaris sp. nov. was proposed with 4228-RoLT (= VKM B-3776 T = UQM 41594 T = BIM B-2058 T) as the type strain. 114 CAZymes including 43 glycoside hydrolases were found to be encoded in the genome of strain 4228-RoLT. Cell-bound and extracellular enzymes of strain 4228-RoLT were active against starch, dextran, mannan, xylan and various kinds of celluloses, with the highest activity against beta-glucan. Altogether, growth experiments, enzymatic activities determination and genomic analysis suggested that T. solaris strain 4228-RoLT could serve as a source of glycosidases suitable for plant biomass hydrolysis.