背景:对腰果/开心果非常敏感的患者对柑橘籽和果胶有过敏反应的风险。
目的:在本研究中,我们试图检查果胶是否被柑橘种子污染,为了鉴定柑橘种子中的罪魁祸首抗原,并评估柑橘种子中过敏原之间的交叉反应性,柑橘果胶,腰果/开心果。
方法:来自橙色种皮的蛋白质,橙色种子胚乳,柠檬种子,葡萄柚种子,柑橘果胶,苹果果胶,提取柚子果胶。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶测定并观察所有提取物中的蛋白质浓度。用Western印迹分析和串联质谱法(MS/MS)测定IgE结合能力,以鉴定柑橘种子和果胶中的罪魁祸首过敏原。
结果:在柑橘种子的受试者中,果胶,腰果过敏,对17-28kDa和28-38kDa之间的条带有很强的IgE反应性。这些条带的MS/MS分析表明存在作为罪魁祸首的变应原的皮苷。Citrin和Anao2都是属于cupin超家族的11S球蛋白,这些蛋白质之间具有显著的同源性。
结论:柑橘果胶可能被柑橘种子污染。Citrin,柑橘种子中一种新发现的过敏原,似乎是柑橘种子和受污染的柑橘果胶中的罪魁祸首抗原。柑橘素与腰果中的Anao2和开心果中的Pisv2高度同源,提示交叉反应的可能性,并为腰果/开心果的共同致敏性提供解释,柑橘种子,还有柑橘果胶.
Patients exquisitely sensitive to cashew/pistachio are at risk for allergic reactions to citrus seeds and pectin.
In this study, we sought to evaluate whether pectin is contaminated with citrus seeds, to identify a culprit antigen in citrus seeds, and to assess for cross-reactivity among allergens in citrus seeds, citrus pectin, and cashew or pistachio.
Proteins from orange seed coats, orange seed endosperms, lemon seeds, grapefruit seeds, citrus pectin, apple pectin, and grapefruit pectin were extracted. Protein concentrations in all extracts were determined and visualized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique. Immunoglobulin E-binding capacity was determined with Western blot analyses and tandem mass spectrometry for the identification of the culprit allergen in citrus seeds and pectin.
In subjects with citrus seed, pectin, and cashew allergies, there was strong immunoglobulin E-reactivity to bands between 17 to 28 kDa and 28 to 38 kDa. The tandem mass spectrometry analysis of these bands indicated the presence of citrin as the culprit allergen. Citrin and Ana o 2 are both 11S globulins belonging to the cupin superfamily, and significant homology was found between these proteins.
Citrus pectin may be contaminated with citrus seeds. Citrin, a newly identified allergen in citrus seeds, seems to be the culprit antigen in citrus seeds and contaminated citrus pectin. Citrin is highly
homologous with Ana o 2 in cashew and Pis v 2 in pistachio, suggesting potential for cross-reactivity and providing an explanation for co-allergenicity of cashew or pistachio, citrus seeds, and citrus pectin.